| T cell-mediated recognition of foreign antigen and the Ia molecule observed by stopped-flow fluorometry. | |
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MedLine Citation:
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PMID: 2457616 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Th cell-mediated rapid recognition of foreign Ag and the Ia molecule was studied using azobenzenearsonate-L-tyrosine (ABA-L-tyrosine)-specific Th cells (I-Ak restricted), foreign Ag (ABA-L-tyrosine), and APC (H-2k). Initial transmembrane signals in Th cell hybridomas (2-45-12) and in Th cell lines (A24-17 or A33-7) were monitored by stopped-flow fluorometry with fluorescent probes. It was found that Th cells recognized foreign Ag within 1 s at 25 degrees C on the APC (B10.BR spleen cells or L cells into which I-Ak genes were transferred). Recognition of foreign Ag and the Ia molecule was shown to deliver the initial signals to Th cell hybridomas and T cell lines. First, Th cells had membrane fluidity increased and then calcium was transported from the external medium into the T cells. The initial transmembrane signals to Th cell hybridomas were inhibited by the addition of an anti-I-Ak mAb. None of the initial signals were observed in the absence of either specific foreign Ag or APC. |
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Authors:
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N Utsunomiya; M Nakanishi; Y Arata; T Yasuda; S Saito; K Koyama; T Tadakuma |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of immunology (Baltimore, Md. : 1950) Volume: 141 ISSN: 0022-1767 ISO Abbreviation: J. Immunol. Publication Date: 1988 Sep |
Date Detail:
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Created Date: 1988-09-26 Completed Date: 1988-09-26 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 2985117R Medline TA: J Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1471-5 Citation Subset: AIM; IM |
Affiliation:
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Faculty of Pharmaceutical Sciences, University of Tokyo, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigen-Presenting Cells / immunology Azo Compounds / immunology* Calcium / metabolism Cell Line Flow Cytometry* / methods Histocompatibility Antigens Class II / immunology* Hybridomas / immunology, metabolism Ion Channels Membrane Fluidity Mice Mice, Inbred AKR Mice, Inbred C57BL Spleen / cytology T-Lymphocytes, Helper-Inducer / immunology*, metabolism Tyrosine / analogs & derivatives*, immunology p-Azobenzenearsonate / analogs & derivatives, immunology* |
| Chemical | |
Reg. No./Substance:
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0/Azo Compounds; 0/Histocompatibility Antigens Class II; 0/Ion Channels; 39927-13-4/azobenzenearsonate-N-acetyl-L-tyrosine; 55520-40-6/Tyrosine; 7334-23-8/p-Azobenzenearsonate; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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