| T cell inflammatory response, Foxp3 and TNFRS18-L regulation of peripheral blood mononuclear cells from patients with nasal polyps-asthma after staphylococcal superantigen stimulation. | |
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MedLine Citation:
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PMID: 20701615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Staphylococcal superantigens may modulate airway inflammatory disease. OBJECTIVE: We assessed the effect of Staphylococcus aureus enterotoxin B (SEB) on T cell activation in patients with nasal polyps and asthma, and its possible link to aspirin hypersensitivity. METHODS: Leucocytes were isolated from five healthy subjects (controls), five asthmatics with nasal polyps without (NP-ATA) and five with aspirin-induced asthma (NP-AIA). Cells were incubated with increasing concentrations of SEB for 4 and 18 h. Release of T(H)1/T(H)2 cytokines was assessed by Cytometric Bead-Array. Foxp3 and TNFRS18-L expression were analysed by qPCR and flow cytometry. RESULTS: After 4 and 18 h, SEB significantly increased IFN-gamma, IL-4, TNF-alpha, IL-5 and IL-2 concentrations in supernatants of both NP polyp groups compared with controls. Baseline Foxp3 was significantly decreased in both NP-asthma groups. Incubation with SEB for 4 h induced a limited up-regulation of Foxp3 in NP-AIA patients, which was switched off consecutively. Foxp3 was significantly up-regulated in the control group after 18 h, but not in the NP-asthmatic groups. In parallel, TNFRS18-L mRNA significantly increased after 18 h in the NP-asthma groups compared with control subjects. This molecule was highly expressed in CD11c(+)CD14(+) cells and its levels increased after 18 and 24 h culture in the NP-asthma patients. CONCLUSION: SEB induces both T(H)1 and T(H)2 pro-inflammatory responses in patients with nasal polyps and asthma regardless of the presence of aspirin hypersensitivity. The nature of this response may be linked to a basal deficiency of Foxp3 observed in the NP-asthmatic patients and/or to the up-regulation of TNFRS18-L on monocytes/dendritic cell precursors. |
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Authors:
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C A Pérez Novo; M Jedrzejczak-Czechowicz; A Lewandowska-Polak; C Claeys; G Holtappels; P Van Cauwenberge; M L Kowalski; C Bachert |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology Volume: 40 ISSN: 1365-2222 ISO Abbreviation: Clin. Exp. Allergy Publication Date: 2010 Sep |
Date Detail:
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Created Date: 2010-08-12 Completed Date: 2011-01-25 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8906443 Medline TA: Clin Exp Allergy Country: England |
Other Details:
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Languages: eng Pagination: 1323-32 Citation Subset: IM |
Affiliation:
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Upper Airways Research Laboratory, Department of Otorhinolaryngology, Ghent University Hospital, De Pintelaan 185, Ghent, Belgium. Claudina.Pereznovo@UGent.be |
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| MeSH Terms | |
Descriptor/Qualifier:
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Adult Aged Asthma, Aspirin-Induced / immunology* Cytokines / biosynthesis Enterotoxins / immunology* Female Forkhead Transcription Factors / metabolism* Humans Leukocytes, Mononuclear Lymphocyte Activation Male Middle Aged Nasal Polyps / immunology* Receptors, Nerve Growth Factor / metabolism* Receptors, Tumor Necrosis Factor / metabolism* Staphylococcus aureus / immunology* Superantigens / immunology* T-Lymphocytes / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Cytokines; 0/Enterotoxins; 0/FOXP3 protein, human; 0/Forkhead Transcription Factors; 0/Receptors, Nerve Growth Factor; 0/Receptors, Tumor Necrosis Factor; 0/Superantigens; 0/TNFRSF18 protein, human; 39424-53-8/enterotoxin B, staphylococcal |
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