Document Detail


T cell depletion of human bone marrow. Comparison of Campath-1 plus complement, anti-T cell ricin A chain immunotoxin, and soybean agglutinin alone or in combination with sheep erythrocytes or immunomagnetic beads.
MedLine Citation:
PMID:  2660360     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The aim of this study was to compare the extent of in vitro T cell depletion and recovery of hematopoietic progenitor cells achieved with five methods of T cell depletion. Bone marrow samples from the same source were treated with monoclonal antibody Campath-1 (CP1) and human complement, XomaZyme-H65 (anti-T cell ricin A chain immunotoxin), or soybean agglutinin (SBA) alone or in combination with sheep erythrocytes (EAET) or a cocktail of immunomagnetic beads (B) directly coated with anti-CD2, anti-CD3, or anti-CD8 monoclonal antibodies. Residual T cells were enumerated by limiting dilution analysis, EAET rosetting, and proliferative responses to phytohemagglutinin. The results of this study demonstrated the following reductions in BM T cells as detected by limiting dilution analysis (mean % control): SBA+B (99.9%), SBA+EAET (99.8%), CP1+C' (99.4%), anti-T cell ricin A chain immunotoxin (99.0%), and SBA alone (94.2%). Neither PHA response nor enumeration of residual EAET rosettes provided discriminating differences in the degree of T cell depletion by treatment method when T cell reductions exceeded 99.0% by LDA. These results demonstrate the ability of CP1+C', XomaZyme-H65, and SBA plus sheep erythrocyte or magnetic bead depletion to achieve a greater than 99% reduction of BM T cells and the importance of limiting dilution analysis in defining differences in T cell numbers when depletion exceeded 99%.
Authors:
J N Frame; N H Collins; T Cartagena; H Waldmann; R J O'Reilly; B Dupont; N A Kernan
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Transplantation     Volume:  47     ISSN:  0041-1337     ISO Abbreviation:  Transplantation     Publication Date:  1989 Jun 
Date Detail:
Created Date:  1989-07-24     Completed Date:  1989-07-24     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0132144     Medline TA:  Transplantation     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  984-8     Citation Subset:  IM    
Affiliation:
Human Immunogenetics Laboratory, Memorial Sloan-Kettering Cancer Center, New York, New York 10021.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies, Monoclonal*
Bone Marrow Transplantation*
Colony-Forming Units Assay
Complement System Proteins
Drug Combinations
Erythrocytes
Hematopoietic Stem Cells / immunology
Humans
Immunotoxins*
Lectins*
Leukocyte Count
Leukocytes, Mononuclear / immunology
Lymphocyte Activation
Lymphocyte Depletion* / methods
Microspheres
Plant Lectins
Rats
Rosette Formation
Sheep
Soybean Proteins*
Soybeans
T-Lymphocytes* / immunology
Grant Support
ID/Acronym/Agency:
CA 08748/CA/NCI NIH HHS; CA 22507/CA/NCI NIH HHS; CA 23766/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Drug Combinations; 0/Immunotoxins; 0/Lectins; 0/Plant Lectins; 0/Soybean Proteins; 0/soybean lectin; 9007-36-7/Complement System Proteins

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