Document Detail


T cell clones from psoriasis skin lesions can promote keratinocyte proliferation in vitro via secreted products.
MedLine Citation:
PMID:  8125129     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Psoriasis vulgaris has been recognized lately as an immunologically mediated inflammatory skin disease. To analyze the pathogenetic role of T lymphocytes in the generation of psoriatic skin lesions, 105 T cell clones (TCC) and 10 T cell lines (TCL) were differentially isolated from dermis and epidermis of psoriatic skin specimens. Supernatants prepared from these T cells were studied for their effects on keratinocyte proliferation in vitro. Conditioned media from 14 of 77 epidermal TCC, 7 of which were CD8+, and from 8 of 28 dermal TCC, 5 of which were CD8+, reproducibly enhanced keratinocyte proliferation, with more pronounced mitogenic activities found in dermal TCC. Another 9 epidermal and 3 dermal TCC did not affect keratinocyte growth and supernatants from the remaining clones, as well as from the 5 epidermal and 5 dermal TCL, inhibited keratinocyte replication to varying degrees. Both mitogenic and suppressive activities were largely abolished by addition of an antiserum to interferon-gamma (IFN-gamma), while addition of epidermal growth factor or irradiated psoriatic TCL had little effect on the activities of the supernatants. These studies reveal that a subpopulation of lesional psoriatic T lymphocytes is capable of enhancing keratinocyte proliferation in vitro via secreted products. Their mitogenic capacity most likely requires IFN-gamma, but the ultimate effect is apparently determined by the presence of additional cytokines. Activation of T cells secreting such combinations of factors in vivo may contribute to the keratinocyte alterations characteristic of psoriatic skin lesions.
Authors:
J C Prinz; B Gross; S Vollmer; P Trommler; I Strobel; M Meurer; G Plewig
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  24     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-04-12     Completed Date:  1994-04-12     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  593-8     Citation Subset:  IM    
Affiliation:
Department of Dermatology, Ludwig-Maximilians-University, Munich, FRG.
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MeSH Terms
Descriptor/Qualifier:
Cell Division
Epidermal Growth Factor / physiology
Epidermis / cytology
Humans
Interferon-gamma / physiology
Keratinocytes / cytology*
Lymphokines / physiology
Mitogens
Psoriasis / immunology*,  pathology
Skin / cytology
T-Lymphocytes / immunology*
Chemical
Reg. No./Substance:
0/Lymphokines; 0/Mitogens; 62229-50-9/Epidermal Growth Factor; 82115-62-6/Interferon-gamma

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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