Document Detail


T cell antigen recognition at the cell membrane.
MedLine Citation:
PMID:  22683645     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
T cell antigen receptors (TCRs) on the surface of T cells bind specifically to particular peptide bound major histocompatibility complexes (pMHCs) presented on the surface of antigen presenting cells (APCs). This interaction is a key event in T cell antigen recognition and activation. Most studies have used surface plasmon resonance (SPR) to measure the in vitro binding kinetics of TCR-pMHC interactions in solution using purified proteins. However, these measurements are not physiologically precise, as both TCRs and pMHCs are membrane-associated molecules which are regulated by their cellular environments. Recently, single-molecule förster resonance energy transfer (FRET) and single-molecule mechanical assays were used to measure the in situ binding kinetics of TCR-pMHC interactions on the surface of live T cells. These studies have provided exciting insights into the biochemical basis of T cell antigen recognition and suggest that TCRs serially engage with a small number of antigens with very fast kinetics in order to maximize TCR signaling and sensitivity.
Authors:
Jun Huang; Christina Meyer; Cheng Zhu
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Review     Date:  2012-06-07
Journal Detail:
Title:  Molecular immunology     Volume:  52     ISSN:  1872-9142     ISO Abbreviation:  Mol. Immunol.     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-07-20     Completed Date:  2012-10-30     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  7905289     Medline TA:  Mol Immunol     Country:  England    
Other Details:
Languages:  eng     Pagination:  155-64     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Microbiology & Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA. junhuanggt@gmail.com
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigen-Presenting Cells / immunology,  metabolism
Antigens, CD3 / immunology*,  metabolism
CD4-Positive T-Lymphocytes / immunology
Cell Membrane / immunology*,  metabolism
Fluorescence Resonance Energy Transfer
Humans
Lymphocyte Activation
Mice
Protein Binding
Receptors, Antigen, T-Cell / chemistry,  immunology*,  metabolism*
Signal Transduction
T-Lymphocytes / immunology*
Grant Support
ID/Acronym/Agency:
R01 GM096187/GM/NIGMS NIH HHS; R01 GM096187/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Antigens, CD3; 0/Receptors, Antigen, T-Cell
Comments/Corrections

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