| T-bet-positive and IRTA1-positive monocytoid B cells differ from marginal zone B cells and epithelial-associated B cells in their antigen profile and topographical distribution. | |
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MedLine Citation:
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PMID: 16079106 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND AND OBJECTIVES: It remains controversial whether splenic and nodal marginal zone B cells, monocytoid -, and epithelial-associated B cells represent separate B-cell subsets or just variants of the same population. To clarify this issue we studied the antigen profile and topographical distribution of these cell types. DESIGN AND METHODS: We studied samples of toxoplasmic lymphadenopathy, lymph nodes with a developed marginal zone, and hyperplastic palatine tonsils. Deparaffinized sections were subjected to antigen-retrieval pre-treatment then incubated with appropriate antibodies. The bound antibodies were made visible using the alkaline phosphatase anti-alkaline phosphatase method with FastRed as the chromogen. RESULTS: We found that i) nodal marginal zone B cells have a similar immunophenotype and topographical distribution to their splenic counterparts, ii) monocytoid B cells differ in their antigen profile (presence of T-bet, IRTA1, CD75, CD45RA, absence of BCL-2 , CD21, CD27) from splenic and nodal marginal zone B cells and more closely resemble epithelial-associated B cells (presence of IRTA-1, CD45RA, partial expression of T-bet, CD75, absence of CD21, CD27). Monocytoid B cells were mostly not found in nodal marginal zones when a marginal zone was developed, but were seen in areas adjacent to marginal zones and occasionally in germinal centers. INTERPRETATION AND CONCLUSIONS: Collectively, our results indicate that monocytoid B cells represent a distinct differentiated B-cell subset, and provide a solid basis for isolation and functional investigations of the cell types studied, and for revising the hitherto inadequate classification of nodal marginal zone lymphomas. |
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Authors:
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Korinna Jöhrens; Yoshihiko Shimizu; Ioannis Anagnostopoulos; Stefan Schiffmann; Enrico Tiacci; Brunangelo Falini; Harald Stein |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Haematologica Volume: 90 ISSN: 1592-8721 ISO Abbreviation: Haematologica Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-04 Completed Date: 2006-08-01 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 0417435 Medline TA: Haematologica Country: Italy |
Other Details:
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Languages: eng Pagination: 1070-7 Citation Subset: IM |
Affiliation:
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Institute for Pathology, Charité, Campus Benjamin Franklin, Medical University Berlin, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Antigens, CD
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analysis B-Lymphocyte Subsets / immunology* B-Lymphocytes / immunology* Humans Lymph Nodes / immunology, pathology Palatine Tonsil / immunology, pathology Receptors, Cell Surface / analysis* Receptors, Fc / analysis* Spleen / immunology*, pathology |
| Chemical | |
Reg. No./Substance:
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0/Antigens, CD; 0/FCRL4 protein, human; 0/Receptors, Cell Surface; 0/Receptors, Fc |
| Comments/Corrections | |
Comment In:
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Haematologica. 2005 Aug;90(8):1011B
[PMID:
16079093
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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