Document Detail

T-bet-positive and IRTA1-positive monocytoid B cells differ from marginal zone B cells and epithelial-associated B cells in their antigen profile and topographical distribution.
MedLine Citation:
PMID:  16079106     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND OBJECTIVES: It remains controversial whether splenic and nodal marginal zone B cells, monocytoid -, and epithelial-associated B cells represent separate B-cell subsets or just variants of the same population. To clarify this issue we studied the antigen profile and topographical distribution of these cell types. DESIGN AND METHODS: We studied samples of toxoplasmic lymphadenopathy, lymph nodes with a developed marginal zone, and hyperplastic palatine tonsils. Deparaffinized sections were subjected to antigen-retrieval pre-treatment then incubated with appropriate antibodies. The bound antibodies were made visible using the alkaline phosphatase anti-alkaline phosphatase method with FastRed as the chromogen. RESULTS: We found that i) nodal marginal zone B cells have a similar immunophenotype and topographical distribution to their splenic counterparts, ii) monocytoid B cells differ in their antigen profile (presence of T-bet, IRTA1, CD75, CD45RA, absence of BCL-2 , CD21, CD27) from splenic and nodal marginal zone B cells and more closely resemble epithelial-associated B cells (presence of IRTA-1, CD45RA, partial expression of T-bet, CD75, absence of CD21, CD27). Monocytoid B cells were mostly not found in nodal marginal zones when a marginal zone was developed, but were seen in areas adjacent to marginal zones and occasionally in germinal centers. INTERPRETATION AND CONCLUSIONS: Collectively, our results indicate that monocytoid B cells represent a distinct differentiated B-cell subset, and provide a solid basis for isolation and functional investigations of the cell types studied, and for revising the hitherto inadequate classification of nodal marginal zone lymphomas.
Korinna Jöhrens; Yoshihiko Shimizu; Ioannis Anagnostopoulos; Stefan Schiffmann; Enrico Tiacci; Brunangelo Falini; Harald Stein
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Haematologica     Volume:  90     ISSN:  1592-8721     ISO Abbreviation:  Haematologica     Publication Date:  2005 Aug 
Date Detail:
Created Date:  2005-08-04     Completed Date:  2006-08-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  0417435     Medline TA:  Haematologica     Country:  Italy    
Other Details:
Languages:  eng     Pagination:  1070-7     Citation Subset:  IM    
Institute for Pathology, Charité, Campus Benjamin Franklin, Medical University Berlin, Germany.
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MeSH Terms
Antigens, CD / analysis
B-Lymphocyte Subsets / immunology*
B-Lymphocytes / immunology*
Lymph Nodes / immunology,  pathology
Palatine Tonsil / immunology,  pathology
Receptors, Cell Surface / analysis*
Receptors, Fc / analysis*
Spleen / immunology*,  pathology
Reg. No./Substance:
0/Antigens, CD; 0/FCRL4 protein, human; 0/Receptors, Cell Surface; 0/Receptors, Fc
Comment In:
Haematologica. 2005 Aug;90(8):1011B   [PMID:  16079093 ]

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