Document Detail


T Regulatory Cells and TH17 Cells in Peri-Silicone Implant Capsular Fibrosis.
MedLine Citation:
PMID:  22286447     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
BACKGROUND: : The authors investigated the immunological mechanisms underlying extensive peri-silicone implant capsule formation, one of the most frequent postoperative complications in patients receiving silicone mammary implants.
METHODS: : The authors studied immune response activation by phenotypic and functional characterization of lymphocytes accumulated within this tissue. Intracapsular lymphoid cells and autologous peripheral blood mononuclear cells were isolated and analyzed by flow cytometry. The proportion of T regulatory cells (CD4CD25Foxp3CD127), the cytokine profiles, and the T cell receptor repertoire of these cells were examined. Intracapsular T regulatory cells were then further analyzed by immunohistochemistry and functional suppression assays.
RESULTS: : In comparison with peripheral blood, the cellular composition of intracapsular lymphocytes showed a predominance of CD4 cells. Intracapsular T cells predominantly produced interleukin-17, interleukin-6, interleukin-8, transforming growth factor-β1, and interferon-γ, suggesting a TH1/TH17-weighted local immune response. Intracapsular T cells displayed a restricted T cell receptor α/β repertoire. The intact suppressive potential of T regulatory cells was demonstrated in crossover experiments with activated peripheral T cells. They did not, however, suppress intracapsular T cells. Interestingly, ratios of intracapsular T regulatory cells were inversely proportional to the clinical degree of capsular fibrosis.
CONCLUSION: : The authors' results indicate that silicone implants trigger a specific, antigen-driven local immune response through activated TH1/TH17 cells, suggesting that ensuing fibrosis is promoted by the production of profibrotic cytokines as a consequence of faltering function of local T regulatory cells.
Authors:
Dolores Wolfram; Evelyn Rabensteiner; Cecilia Grundtman; Günther Böck; Christina Mayerl; Walther Parson; Giovanni Almanzar; Carlo Hasenöhrl; Hildegunde Piza-Katzer; Georg Wick
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Plastic and reconstructive surgery     Volume:  129     ISSN:  1529-4242     ISO Abbreviation:  Plast. Reconstr. Surg.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1306050     Medline TA:  Plast Reconstr Surg     Country:  United States    
Other Details:
Languages:  eng     Pagination:  327e-37e     Citation Subset:  AIM; IM    
Affiliation:
Innsbruck, Austria From the Department of Plastic and Reconstructive Surgery, the Laboratory of Autoimmunity, Division of Experimental Pathophysiology and Immunology, Biocenter, and the Institute for Legal Medicine, Innsbruck Medical University; and Excellentbeauty.
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