| T Cell Epitopes of the Timothy Grass Pollen Allergen Phl p 5 of Mice and Men and the Detection of Allergen-Specific T Cells Using Class II Ultimers. | |
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MedLine Citation:
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PMID: 22472723 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Knowledge of allergen-specific T cell epitopes is a prerequisite not only for therapeutic approaches but also for elucidating immunological mechanisms of type I allergy. Ex vivo detection of allergen-specific T cells using class II tetramer technology has become an important tool for investigating immune responses in atopic and healthy individuals. Methods: Using (3)H-thymidine incorporation assays, T cell epitopes specific for the major timothy grass pollen allergen Phl p 5.0101 were mapped in 11 allergic donors and two different mouse strains. Different protocols for expansion/restimulation of T cells from the blood of allergic donors and detection of allergen-specific T cells by Class II Ultimer staining were evaluated. Results: We identified several new Phl p 5.0101 class II T cell epitopes in allergic patients and confirmed previously published ones. Additionally, we discovered the major T cell epitopes in BALB/c and C57BL/6 mice. Using a novel Class II Ultimer, we detected epitope-specific T cells expanded from the blood of an allergic donor. Conclusions: Epitope mapping of Phl p 5.0101 revealed an immunodominant epitope in BALB/c and C57BL/6 mice and an immunodominant region in humans (amino acids 259-282), which was recognized by 8 out of 11 allergic donors. Detection of Phl p 5-specific T cells was demonstrated using a Class II Ultimer specific for epitope 196-210. Successful detection of ultimer-positive T cells was strongly dependent on a resting phase after in vitro expansion. |
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Authors:
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Angelika Stoecklinger; Sandra Scheiblhofer; Elisabeth Roesler; Andreas Lang; Gerd Fastner; Felix Sedlmayer; Roland Lang; Martin Danzer; Josef Thalhamer; Richard Weiss |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-4-03 |
Journal Detail:
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Title: International archives of allergy and immunology Volume: 158 ISSN: 1423-0097 ISO Abbreviation: - Publication Date: 2012 Apr |
Date Detail:
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Created Date: 2012-4-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9211652 Medline TA: Int Arch Allergy Immunol Country: - |
Other Details:
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Languages: ENG Pagination: 326-334 Citation Subset: - |
Copyright Information:
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Copyright © 2012 S. Karger AG, Basel. |
Affiliation:
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Christian Doppler Laboratory for Allergy Diagnosis and Therapy, Department of Molecular Biology, University of Salzburg, Salzburg, Austria. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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