Document Detail


Systems biology of the cell cycle of Saccharomyces cerevisiae: From network mining to system-level properties.
MedLine Citation:
PMID:  19465107     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Following a brief description of the operational procedures of systems biology (SB), the cell cycle of budding yeast is discussed as a successful example of a top-down SB analysis. After the reconstruction of the steps that have led to the identification of a sizer plus timer network in the G1 to S transition, it is shown that basic functions of the cell cycle (the setting of the critical cell size and the accuracy of DNA replication) are system-level properties, detected only by integrating molecular analysis with modelling and simulation of their underlying networks. A detailed network structure of a second relevant regulatory step of the cell cycle, the exit from mitosis, derived from extensive data mining, is constructed and discussed. To reach a quantitative understanding of how nutrients control, through signalling, metabolism and transcription, cell growth and cycle is a very relevant aim of SB. Since we know that about 900 gene products are required for cell cycle execution and control in budding yeast, it is quite clear that a purely systematic approach would require too much time. Therefore lines for a modular SB approach, which prioritises molecular and computational investigations for faster cell cycle understanding, are proposed. The relevance of the insight coming from the cell cycle SB studies in developing a new framework for tackling very complex biological processes, such as cancer and aging, is discussed.
Authors:
Lilia Alberghina; Paola Coccetti; Ivan Orlandi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Review     Date:  2009-05-22
Journal Detail:
Title:  Biotechnology advances     Volume:  27     ISSN:  1873-1899     ISO Abbreviation:  Biotechnol. Adv.     Publication Date:    2009 Nov-Dec
Date Detail:
Created Date:  2009-10-12     Completed Date:  2010-01-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8403708     Medline TA:  Biotechnol Adv     Country:  England    
Other Details:
Languages:  eng     Pagination:  960-78     Citation Subset:  IM    
Affiliation:
Department of Biotechnology and Biosciences, University of Milano-Bicocca, P.zza della Scienza 2, 20126 Milano, Italy. lilia.alberghina@unimib.it
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MeSH Terms
Descriptor/Qualifier:
Cell Cycle*
Saccharomyces cerevisiae / cytology*
Systems Biology*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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