Document Detail

Systemic ovalbumin sensitization downregulates norepinephrine uptake by rabbit aortic smooth muscle cells.
MedLine Citation:
PMID:  12444035     Owner:  NLM     Status:  MEDLINE    
Norepinephrine (NE) concentration at alpha-adrenergic receptors is partially regulated by steroid-sensitive, extraneuronal catecholamine uptake (uptake-2). Because alpha(1)-adrenergic agonist- and glucocorticosteroid (GS)-induced bronchial vasoconstriction is enhanced in individuals with asthma, atopy could be associated with decreased uptake-2 by vascular smooth muscle cells (SMCs). We therefore evaluated whether NE uptake and its specific transporter messenger RNA (mRNA) were reduced in aortic SMCs of rabbits systemically sensitized with ovalbumin (OVA). NE uptake was measured using a semiquantitative fluorescence microscopic method. Corticosterone and O-methyl-isoprenaline, but not desipramine, co-incubation (1 micro M each) for 20 min decreased NE uptake into SMCs, an inhibitor profile indicative of extraneuronal monoamine transporter (EMT). In OVA-sensitized rabbits, NE uptake was 25.9 +/- 4.5% (mean +/- SEM) lower than in control animals (P < 0.05). Sensitized serum had no effect on NE uptake into naive SMCs. EMT mRNA expression was measured in aortic smooth muscle, using multiplex reverse transcriptase-polymerase chain reaction. In OVA-sensitized rabbits, expression was 61.1 +/- 16.4% lower than in control animals (P < 0.05). These data demonstrate that NE uptake by aortic SMCs is impaired in atopic rabbits, and associated with a decreased transporter mRNA expression. The same mechanism may operate in bronchial arteries in individuals with asthma.
Gabor Horvath; Aliza Torbati; Gregory E Conner; Matthias Salathe; Adam Wanner
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of respiratory cell and molecular biology     Volume:  27     ISSN:  1044-1549     ISO Abbreviation:  Am. J. Respir. Cell Mol. Biol.     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-11-21     Completed Date:  2003-01-08     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8917225     Medline TA:  Am J Respir Cell Mol Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  746-51     Citation Subset:  IM    
Division of Pulmonary and Critical Care Medicine, and Department of Cell Biology and Anatomy, University of Miami School of Medicine, Miami, Florida 33101, USA.
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MeSH Terms
Adrenergic alpha-Agonists / pharmacokinetics*
Aorta / cytology,  metabolism
Asthma / metabolism
Carrier Proteins / genetics*,  metabolism
Down-Regulation / drug effects,  immunology
Gene Expression / drug effects,  immunology
Muscle, Smooth, Vascular / cytology,  metabolism*
Norepinephrine / pharmacokinetics*
Organic Cation Transport Proteins*
Ovalbumin / immunology,  pharmacology*
RNA, Messenger / analysis
Grant Support
Reg. No./Substance:
0/Adrenergic alpha-Agonists; 0/Carrier Proteins; 0/Organic Cation Transport Proteins; 0/RNA, Messenger; 0/solute carrier family 22 (organic cation transporter), member 3; 51-41-2/Norepinephrine; 9006-59-1/Ovalbumin

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