Document Detail


Systemic lupus erythematosus patients exhibit functional deficiencies of endothelial progenitor cells.
MedLine Citation:
PMID:  18660509     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
OBJECTIVE: SLE is characterized by an increased cardiovascular risk. Since endothelial progenitor cells (EPCs) have been described to serve as a biomarker for the CV risk and are known to be depleted in various diseases, we were interested if SLE would also be associated with altered peripheral EPC levels or functional abnormalities of these cells. METHODS: EPCs were quantified in 31 female SLE patients with different disease activity and in age-matched healthy controls (HCs) by FACS analysis and by colony forming unit (CFU) assay. Furthermore, EPC adhesion and migration capacity were tested. RESULTS: EPC levels were similar in HC and SLE when assessed by FACS (0.045 +/- 0.006% vs 0.036 +/- 0.007% within the lymphocyte gate) and by the CFU assay (18 +/- 3 vs 15 +/- 2 colonies/well). No correlation with disease activity could be observed, but SLE patients treated with chloroquine exhibited significantly decreased EPC levels (0.058 +/- 0.005% without vs 0.024 +/- 0.008% with chloroquine, P < 0.05). Addition of chloroquine to in vitro cultures also led to a decreased colony formation in SLE and in HC. When testing the adhesion and migration capacity of EPC on human umbilical vein endothelial cells (HUVEC), cells from SLE patients had reduced adhesion (19.2 +/- 3.5% vs 36.6 +/- 5.2% EPC/high power field, P < 0.02) and migratory activity (56 +/- 6 cells/random microscopic field in SLE vs 121 +/- 28 in controls, P < 0.02). CONCLUSION: The data reveal that EPCs are significantly affected in SLE. While circulating EPC levels are in the range of HC, they exhibit functional deficiencies that may lead to impaired tissue availability.
Authors:
J Grisar; C W Steiner; M Bonelli; T Karonitsch; I Schwarzinger; G Weigel; G Steiner; J S Smolen
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Publication Detail:
Type:  Journal Article     Date:  2008-07-26
Journal Detail:
Title:  Rheumatology (Oxford, England)     Volume:  47     ISSN:  1462-0332     ISO Abbreviation:  Rheumatology (Oxford)     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-22     Completed Date:  2008-12-04     Revised Date:  2009-04-16    
Medline Journal Info:
Nlm Unique ID:  100883501     Medline TA:  Rheumatology (Oxford)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1476-83     Citation Subset:  AIM; IM    
Affiliation:
Department of Internal Medicine III, Division of Rheumatology, Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna, Austria. johannes.grisar@meduniwien.ac.at
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MeSH Terms
Descriptor/Qualifier:
Adult
Antirheumatic Agents / therapeutic use
Cell Adhesion
Cell Movement / drug effects
Cells, Cultured
Chloroquine / therapeutic use
Cytokines / blood
Endothelial Cells / physiology
Endothelium, Vascular / pathology
Female
Growth Substances / blood
Hematopoietic Stem Cells / drug effects,  physiology*
Humans
Lupus Erythematosus, Systemic / blood*,  drug therapy
Microscopy, Confocal
Chemical
Reg. No./Substance:
0/Antirheumatic Agents; 0/Cytokines; 0/Growth Substances; 54-05-7/Chloroquine
Comments/Corrections
Comment In:
Rheumatology (Oxford). 2009 Apr;48(4):453; author reply 453-4   [PMID:  19164424 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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