Document Detail

Systemic lupus erythematosus patients exhibit functional deficiencies of endothelial progenitor cells.
MedLine Citation:
PMID:  18660509     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: SLE is characterized by an increased cardiovascular risk. Since endothelial progenitor cells (EPCs) have been described to serve as a biomarker for the CV risk and are known to be depleted in various diseases, we were interested if SLE would also be associated with altered peripheral EPC levels or functional abnormalities of these cells. METHODS: EPCs were quantified in 31 female SLE patients with different disease activity and in age-matched healthy controls (HCs) by FACS analysis and by colony forming unit (CFU) assay. Furthermore, EPC adhesion and migration capacity were tested. RESULTS: EPC levels were similar in HC and SLE when assessed by FACS (0.045 +/- 0.006% vs 0.036 +/- 0.007% within the lymphocyte gate) and by the CFU assay (18 +/- 3 vs 15 +/- 2 colonies/well). No correlation with disease activity could be observed, but SLE patients treated with chloroquine exhibited significantly decreased EPC levels (0.058 +/- 0.005% without vs 0.024 +/- 0.008% with chloroquine, P < 0.05). Addition of chloroquine to in vitro cultures also led to a decreased colony formation in SLE and in HC. When testing the adhesion and migration capacity of EPC on human umbilical vein endothelial cells (HUVEC), cells from SLE patients had reduced adhesion (19.2 +/- 3.5% vs 36.6 +/- 5.2% EPC/high power field, P < 0.02) and migratory activity (56 +/- 6 cells/random microscopic field in SLE vs 121 +/- 28 in controls, P < 0.02). CONCLUSION: The data reveal that EPCs are significantly affected in SLE. While circulating EPC levels are in the range of HC, they exhibit functional deficiencies that may lead to impaired tissue availability.
J Grisar; C W Steiner; M Bonelli; T Karonitsch; I Schwarzinger; G Weigel; G Steiner; J S Smolen
Related Documents :
20167859 - Adriamycin nephropathy: a failure of endothelial progenitor cell-induced repair.
17943139 - Erythropoietin, progenitors, and repair.
22915029 - A short-term in vivo experimental model for fuchs endothelial corneal dystrophy.
12829619 - Intravenous transfusion of endothelial progenitor cells reduces neointima formation aft...
17919519 - Apoptosis-associated changes in the glycerophospholipid composition of hematopoietic pr...
15596109 - Endothelial progenitor cells functional characterization.
1683619 - Expression of the cd11/cd18 cell surface adhesion glycoprotein family on alveolar macro...
20505729 - Embolus extravasation is an alternative mechanism for cerebral microvascular recanaliza...
19869049 - Studies on the etiology of heartwater : i. observation of a rickettsia, rickettsia rumi...
Publication Detail:
Type:  Journal Article     Date:  2008-07-26
Journal Detail:
Title:  Rheumatology (Oxford, England)     Volume:  47     ISSN:  1462-0332     ISO Abbreviation:  Rheumatology (Oxford)     Publication Date:  2008 Oct 
Date Detail:
Created Date:  2008-09-22     Completed Date:  2008-12-04     Revised Date:  2009-04-16    
Medline Journal Info:
Nlm Unique ID:  100883501     Medline TA:  Rheumatology (Oxford)     Country:  England    
Other Details:
Languages:  eng     Pagination:  1476-83     Citation Subset:  AIM; IM    
Department of Internal Medicine III, Division of Rheumatology, Ludwig Boltzmann Institute for Rheumatology and Balneology, Vienna, Austria.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antirheumatic Agents / therapeutic use
Cell Adhesion
Cell Movement / drug effects
Cells, Cultured
Chloroquine / therapeutic use
Cytokines / blood
Endothelial Cells / physiology
Endothelium, Vascular / pathology
Growth Substances / blood
Hematopoietic Stem Cells / drug effects,  physiology*
Lupus Erythematosus, Systemic / blood*,  drug therapy
Microscopy, Confocal
Reg. No./Substance:
0/Antirheumatic Agents; 0/Cytokines; 0/Growth Substances; 54-05-7/Chloroquine
Comment In:
Rheumatology (Oxford). 2009 Apr;48(4):453; author reply 453-4   [PMID:  19164424 ]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Fertility preservation treatment for young women with autoimmune diseases facing treatment with gona...
Next Document:  The value of synovial cytokine expression in predicting the clinical response to TNF antagonist ther...