Document Detail


Systemic effects of angiotensin III in conscious dogs during acute double blockade of the renin-angiotensin-aldosterone-system.
MedLine Citation:
PMID:  16948800     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS: The study was designed to determine (i) whether the effects of angiotensin III (AngIII) are similar to those of angiotensin II (AngII) at identical plasma concentrations and (ii) whether AngIII operates solely through AT1- receptors. METHODS: Angiotensin II (3 pmol kg(-1) min(-1)-3.1 ng kg(-1) min(-1)) or AngIII (15 pmol kg(-1) min(-1)-14 ng kg(-1) min(-1)) was infused i.v. during acute inhibition of angiotensin converting enzyme (enalaprilate; 2 mg kg(-1)) and of aldosterone (canrenoate; 6 mg kg(-1) plus 1 mg kg(-1) h(-1)). Arterial plasma concentrations of angiotensins were determined by radioimmunoassay using a cross-reacting antibody to AngII. During ongoing peptide infusion, candesartan (2 mg kg(-1)) was administered to block the AT1-receptors. RESULTS: Angiotensin immunoactivity in plasma increased to 60 +/- 10 pg mL(-1) during infusion of AngII or infusion of AngIII. AngII significantly increased mean arterial blood pressure (+14 +/- 4 mmHg) and plasma aldosterone by 79% (+149 +/- 17 pg mL(-1)) and reduced plasma renin activity and sodium excretion (-41 +/- 16 mIU L(-1) and -46 +/- 6 micromol min(-1) respectively). AngIII mimicked these effects and the magnitude of AngIII responses was statistically indistinguishable from those of AngII. All measured effects of both peptides were blocked by candesartan. CONCLUSION: At the present arterial plasma concentrations, AngIII is equipotent to AngII with regard to effects on blood pressure, aldosterone secretion and renal functions, and these AngIII effects are mediated through AT1- receptors. The metabolic clearance rate of AngIII is five times that of AngII.
Authors:
I Gammelgaard; S Wamberg; P Bie
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta physiologica (Oxford, England)     Volume:  188     ISSN:  1748-1708     ISO Abbreviation:  Acta Physiol (Oxf)     Publication Date:  2006 Oct 
Date Detail:
Created Date:  2006-09-04     Completed Date:  2007-06-29     Revised Date:  2009-02-03    
Medline Journal Info:
Nlm Unique ID:  101262545     Medline TA:  Acta Physiol (Oxf)     Country:  England    
Other Details:
Languages:  eng     Pagination:  129-38     Citation Subset:  IM    
Affiliation:
Department of Physiology and Pharmacology, Institute of Medical Biology, University of Southern Denmark, Odense, Denmark.
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MeSH Terms
Descriptor/Qualifier:
Aldosterone / blood
Angiotensin II / blood,  pharmacology
Angiotensin II Type 1 Receptor Blockers / pharmacology
Angiotensin III / blood,  pharmacology*
Angiotensin-Converting Enzyme Inhibitors / pharmacology
Animals
Atrial Natriuretic Factor / blood
Benzimidazoles / pharmacology
Blood Pressure / drug effects
Dogs
Dose-Response Relationship, Drug
Enalaprilat / pharmacology
Female
Glomerular Filtration Rate / drug effects,  physiology
Metabolic Clearance Rate
Receptor, Angiotensin, Type 1 / physiology
Renin / blood
Renin-Angiotensin System / drug effects,  physiology*
Tetrazoles / pharmacology
Chemical
Reg. No./Substance:
0/Angiotensin II Type 1 Receptor Blockers; 0/Angiotensin-Converting Enzyme Inhibitors; 0/Benzimidazoles; 0/Receptor, Angiotensin, Type 1; 0/Tetrazoles; 11128-99-7/Angiotensin II; 12687-51-3/Angiotensin III; 139481-59-7/candesartan; 52-39-1/Aldosterone; 84680-54-6/Enalaprilat; 85637-73-6/Atrial Natriuretic Factor; EC 3.4.23.15/Renin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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