| Systemic neutrophil activation in a mouse model of ischemic stroke and reperfusion. | |
| | |
MedLine Citation:
|
PMID: 21044968 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
As a natural response to injury and disease, neutrophils activate, adhere to the microvasculature, migrate into brain tissue, and release toxic substances such as reactive oxygen species and proteases. This neutrophil response occurs when blood flow is returned to brain tissue (reperfusion) after ischemic stroke. Thus, the presence of activated systemic neutrophils increases the potential for tissue injury during reperfusion after ischemic stroke. Although experiments in rat models suggest that activated neutrophils play a pivotal role in cerebral ischemia reperfusion injury, little is known about systemic neutrophil activation during reperfusion following ischemic stroke in a mouse model. The purpose of this study was to characterize systemic leukocyte responses and neutrophil CD11b expression 15-min and 24-hr post-reperfusion in a mouse model of ischemic stroke. The intraluminal filament method of transient middle cerebral artery occlusion (tMCAO) with reperfusion or a sham procedure was performed in male C57Bl/6 mice. Automated leukocyte counts and manual white blood cell (WBC) differential counts were measured. Flow cytometry was used to assess systemic neutrophil surface CD11b expression. The data suggest that the damaging potential of systemic neutrophil activation begins as early as 15 min and remains evident at 24 hr after the initiation of reperfusion. In addition, because transgenic mouse models, bred on a C57Bl/6 background, are increasingly used to elucidate single mechanisms of reperfusion injury after ischemic stroke, findings from this study are foundational for future investigations examining the damaging potential of neutrophil responses post-reperfusion after ischemic stroke in genetically altered mouse models within this background strain. |
| | |
Authors:
|
Helena Morrison; Dana McKee; Leslie Ritter |
Related Documents
:
|
7702538 - Decreased interstitial glucose and transmural gradient in lactate during ischemia. 15221348 - Antiarrhythmic effect of ischemic preconditioning during low-flow ischemia. the role of... 19331518 - Changes in blood flow, oxygen tension, action potentials, and vascular permeability ind... 18484908 - Raman spectroscopy and fluorescence analysis in investigation of the protective action ... 2880628 - Effects of alpha-adrenoceptor agonists on cardiac output and its regional distribution ... 8683668 - Doppler velocimetry of the fetal middle cerebral and renal arteries: interobserver reli... |
Publication Detail:
|
Type: Journal Article Date: 2010-11-01 |
Journal Detail:
|
Title: Biological research for nursing Volume: 13 ISSN: 1552-4175 ISO Abbreviation: Biol Res Nurs Publication Date: 2011 Apr |
Date Detail:
|
Created Date: 2011-03-16 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9815758 Medline TA: Biol Res Nurs Country: United States |
Other Details:
|
Languages: eng Pagination: 154-63 Citation Subset: IM; N |
Affiliation:
|
College of Nursing, University of Arizona, Tucson, AZ, USA. hmorrison@nursing.arizona.edu. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Gene expression in first trimester preeclampsia placenta.
Next Document: Major depressive disorder and insulin resistance in nondiabetic young adults in the United States: t...