Document Detail

Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.
MedLine Citation:
PMID:  23142872     Owner:  NLM     Status:  MEDLINE    
We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.
Lance Martin; Matthias Meier; Shawn M Lyons; Rene V Sit; William F Marzluff; Stephen R Quake; Howard Y Chang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-11
Journal Detail:
Title:  Nature methods     Volume:  9     ISSN:  1548-7105     ISO Abbreviation:  Nat. Methods     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-02-06     Revised Date:  2014-05-21    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1192-4     Citation Subset:  IM    
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MeSH Terms
Base Sequence
Gene Library
Glutathione Transferase
Microfluidics / methods*
Nucleotide Motifs*
RNA / chemistry*
RNA Probes
Grant Support
R01 GM029832/GM/NIGMS NIH HHS; R01 HG004361/HG/NHGRI NIH HHS; R01-HG004361/HG/NHGRI NIH HHS; R01GM29832/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute
Reg. No./Substance:
0/RNA Probes; 63231-63-0/RNA; EC Transferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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