Document Detail


Systematic reconstruction of RNA functional motifs with high-throughput microfluidics.
MedLine Citation:
PMID:  23142872     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We present RNA-mechanically induced trapping of molecular interactions (RNA-MITOMI), a microfluidic platform that allows integrated synthesis and functional assays for programmable RNA libraries. The interaction of a comprehensive library of RNA mutants with stem-loop-binding protein precisely defined the RNA structural and sequence features that govern affinity. The functional motif reconstructed in a single experiment on our platform uncovers new binding specificities and enriches interpretation of phylogenetic data.
Authors:
Lance Martin; Matthias Meier; Shawn M Lyons; Rene V Sit; William F Marzluff; Stephen R Quake; Howard Y Chang
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-11-11
Journal Detail:
Title:  Nature methods     Volume:  9     ISSN:  1548-7105     ISO Abbreviation:  Nat. Methods     Publication Date:  2012 Dec 
Date Detail:
Created Date:  2012-12-11     Completed Date:  2013-02-06     Revised Date:  2014-05-21    
Medline Journal Info:
Nlm Unique ID:  101215604     Medline TA:  Nat Methods     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1192-4     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Gene Library
Glutathione Transferase
Humans
Microfluidics / methods*
Nucleotide Motifs*
Phylogeny
RNA / chemistry*
RNA Probes
Grant Support
ID/Acronym/Agency:
R01 GM029832/GM/NIGMS NIH HHS; R01 HG004361/HG/NHGRI NIH HHS; R01-HG004361/HG/NHGRI NIH HHS; R01GM29832/GM/NIGMS NIH HHS; //Howard Hughes Medical Institute
Chemical
Reg. No./Substance:
0/RNA Probes; 63231-63-0/RNA; EC 2.5.1.18/Glutathione Transferase
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A toolkit and benchmark study for FRET-restrained high-precision structural modeling.
Next Document:  Two-photon optogenetics of dendritic spines and neural circuits.