| Systematic determination of telomerase activity and telomerase length during the progression of human breast cancer in cell culture models. | |
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MedLine Citation:
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PMID: 9494546 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The purpose of the study was to determine systematically the expression of telomerase activity and the length of telomere repeat arrays by utilizing two different cell culture models that derive from normal individual donors, and probably represent various stages of human breast oncogenesis in cell culture. The models consist of mortal, non-tumorigenic immortal and tumorigenic immortal human mammary epithelial cell (MEC) lines. Using a recently developed polymerase chain reaction (PCR)-based telomeric repeat amplification protocol (TRAP) assay, telomerase activity was undetectable in mortal MEC cells. In contrast, the immortal MEC that were nontumorigenic or tumorigenic in immunosuppressed athymic mice, showed telomerase activity. The absence of telomerase activity in mortal and its presence in both non-tumorigenic and tumorigenic immortal cell lines did not reflect their proliferative rate, as demonstrated by the similar pattern and intensity of reactivity of these cell lines with anti-Ki 67 antibody which recognizes a human nuclear cell proliferation--associated antigen. Southern blot analyses of Hinf I-digested genomic DNA hybridized with a (TTAGGG)4 probe revealed arrays of telomeric repeat lengths ranging from 3 to 5, 3.5 to 9, 3.2 to 9 or 3 to 15 kilobase pair (kbp) for mortal, nontumorigenic immortal, and tumorigenic immortal or established MEC lines respectively. These results suggest that telomerase activity and stable telomeric repeat lengths may be a molecular phenotype of the early stages in the progression of breast cancer. |
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Authors:
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S A Imam; M S Kim; L Anker; R H Datar; R E Law; C R Taylor |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Anticancer research Volume: 17 ISSN: 0250-7005 ISO Abbreviation: Anticancer Res. Publication Date: 1997 Nov-Dec |
Date Detail:
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Created Date: 1998-03-26 Completed Date: 1998-03-26 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8102988 Medline TA: Anticancer Res Country: GREECE |
Other Details:
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Languages: eng Pagination: 4435-41 Citation Subset: IM |
Affiliation:
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Department of Pathology, University of Southern California, School of Medicine, Los Angeles 90033, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Breast / cytology, enzymology Breast Neoplasms / enzymology*, genetics, pathology* Cell Line, Transformed Cells, Cultured DNA Probes Epithelial Cells / cytology, enzymology Female Gene Amplification Humans Ki-67 Antigen / analysis Mice Mice, Nude Repetitive Sequences, Nucleic Acid Telomerase / genetics*, metabolism* Transplantation, Heterologous Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/DNA Probes; 0/Ki-67 Antigen; EC 2.7.7.49/Telomerase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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