Document Detail


System response of metabolic networks in Chlamydomonas reinhardtii to total available ammonium.
MedLine Citation:
PMID:  22787274     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Drastic alterations in macronutrients are known to cause large changes in biochemistry and gene expression in the photosynthetic alga Chlamydomonas reinhardtii. However, metabolomic and proteomic responses to subtle reductions in macronutrients have not yet been studied. When ammonium levels were reduced by 25-100% compared with control cultures, ammonium uptake and growth rates were not affected at 25% or 50% nitrogen-reduction for 28 h. However, primary metabolism and enzyme expression showed remarkable changes at acute conditions (4 h and 10 h after ammonium reduction) compared with chronic conditions (18 h and 28 h time points). Responses of 145 identified metabolites were quantified using gas chromatography-time of flight mass spectrometry; 495 proteins (including 187 enzymes) were monitored using liquid chromatography-ion trap mass spectrometry with label-free spectral counting. Stress response and carbon assimilation processes (Calvin cycle, acetate uptake and chlorophyll biosynthesis) were altered first, in addition to increase in enzyme contents for lipid biosynthesis and accumulation of short chain free fatty acids. Nitrogen/carbon balance metabolism was found changed only under chronic conditions, for example in the citric acid cycle and amino acid metabolism. Metabolism in Chlamydomonas readily responds to total available media nitrogen with temporal increases in short-chain free fatty acids and turnover of internal proteins, long before nitrogen resources are depleted.
Authors:
Do Yup Lee; Jeong-Jin Park; Dinesh K Barupal; Oliver Fiehn
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2012-07-10
Journal Detail:
Title:  Molecular & cellular proteomics : MCP     Volume:  11     ISSN:  1535-9484     ISO Abbreviation:  Mol. Cell Proteomics     Publication Date:  2012 Oct 
Date Detail:
Created Date:  2012-10-01     Completed Date:  2013-02-13     Revised Date:  2013-10-11    
Medline Journal Info:
Nlm Unique ID:  101125647     Medline TA:  Mol Cell Proteomics     Country:  United States    
Other Details:
Languages:  eng     Pagination:  973-88     Citation Subset:  IM    
Affiliation:
University of California, Davis Genome Center, Davis, California 95616, USA.
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MeSH Terms
Descriptor/Qualifier:
Acetic Acid / metabolism
Amino Acids / metabolism
Carbon / metabolism*
Chlamydomonas reinhardtii / drug effects,  genetics,  metabolism*
Chlorophyll / biosynthesis
Citric Acid Cycle / drug effects,  physiology
Fatty Acids / biosynthesis
Gas Chromatography-Mass Spectrometry
Gene Expression Regulation, Plant / drug effects*
Metabolic Networks and Pathways / drug effects*,  physiology
Metabolomics
Nitrogen / metabolism*
Photosynthesis / drug effects,  physiology
Quaternary Ammonium Compounds / metabolism*,  pharmacology
Chemical
Reg. No./Substance:
0/Amino Acids; 0/Fatty Acids; 0/Quaternary Ammonium Compounds; 1406-65-1/Chlorophyll; 64-19-7/Acetic Acid; 7440-44-0/Carbon; 7727-37-9/Nitrogen
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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