Document Detail


System-level biochip for impedance sensing and programmable manipulation of bladder cancer cells.
MedLine Citation:
PMID:  22346685     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
This paper develops a dielectrophoretic (DEP) chip with multi-layer electrodes and a micro-cavity array for programmable manipulations of cells and impedance measurement. The DEP chip consists of an ITO top electrode, flow chamber, middle electrode on an SU-8 surface, micro-cavity arrays of SU-8 and distributed electrodes at the bottom of the micro-cavity. Impedance sensing of single cells could be performed as follows: firstly, cells were trapped in a micro-cavity array by negative DEP force provided by top and middle electrodes; then, the impedance measurement for discrimination of different stage of bladder cancer cells was accomplished by the middle and bottom electrodes. After impedance sensing, the individual releasing of trapped cells was achieved by negative DEP force using the top and bottom electrodes in order to collect the identified cells once more. Both cell manipulations and impedance measurement had been integrated within a system controlled by a PC-based LabVIEW program. In the experiments, two different stages of bladder cancer cell lines (grade III: T24 and grade II: TSGH8301) were utilized for the demonstration of programmable manipulation and impedance sensing; as the results show, the lower-grade bladder cancer cells (TSGH8301) possess higher impedance than the higher-grade ones (T24). In general, the multi-step manipulations of cells can be easily programmed by controlling the electrical signal in our design, which provides an excellent platform technology for lab-on-a-chip (LOC) or a micro-total-analysis-system (Micro TAS).
Authors:
Cheng-Hsin Chuang; Yao-Wei Huang; Yao-Tung Wu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-11-23
Journal Detail:
Title:  Sensors (Basel, Switzerland)     Volume:  11     ISSN:  1424-8220     ISO Abbreviation:  Sensors (Basel)     Publication Date:  2011  
Date Detail:
Created Date:  2012-02-20     Completed Date:  2012-07-12     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  101204366     Medline TA:  Sensors (Basel)     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  11021-35     Citation Subset:  IM    
Affiliation:
Department of Mechanical Engineering, Southern Taiwan University, Tainan 71005, Taiwan. chchuang@mail.stut.edu.tw
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MeSH Terms
Descriptor/Qualifier:
Algorithms
Cell Line, Tumor
Computer Simulation
Electric Impedance
Electromagnetic Phenomena
Electrophoresis, Microchip / instrumentation*,  methods
Electrophysiological Phenomena / physiology
Epoxy Compounds / chemistry
Gold / chemistry
Humans
Microarray Analysis / instrumentation*,  methods
Microelectrodes
Microscopy, Electron, Scanning
Neoplasm Grading
Polymers / chemistry
Software
Urinary Bladder Neoplasms / pathology,  physiopathology*
User-Computer Interface
Chemical
Reg. No./Substance:
0/Epoxy Compounds; 0/Polymers; 0/SU-8 compound; 7440-57-5/Gold
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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