| System A amino acid transport in incubated muscle: effects of insulin and acute uremia. | |
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MedLine Citation:
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PMID: 3524266 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The insulin-stimulated increase in amino acid uptake in most cells and tissues involves stimulation of system A transport. In muscle the probes used to study this process have not been specific, making it difficult to determine whether system A is abnormal in insulin-resistant states, such as acute renal failure (ARF). To circumvent this problem, we studied 2-(methylamino) isobutyrate (MeAIB) transport. Its specificity for system A in incubated rat epitrochlearis muscles was documented by showing its uptake by only one carrier that is sodium dependent and insulin responsive and that exhibits adaptive regulation in response to starvation. Using this specific probe we determined whether insulin-stimulated amino acid transport by system A is impaired by ARF. MeAIB uptake was linear for 3 h in muscles of ARF and sham-operated (SO) rats. In the absence (basal) or presence of insulin, MeAIB uptake was significantly lower in ARF, yet the stimulation by insulin was similar in both groups. Likewise, the insulin dose-response relationship confirmed that physiological levels of insulin (less than or equal to 10(2) microU/ml) increased transport by a similar degree. At greater than or equal to 10(2) microU/ml insulin there was a plateau in MeAIB transport in ARF but not in SO muscles. Thus basal system A transport is depressed in ARF, but the stimulation of system A by physiological levels of insulin is preserved. At pharmacological levels of insulin system A transport is impaired by ARF. |
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Authors:
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B J Maroni; G Karapanos; W E Mitch |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: The American journal of physiology Volume: 251 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1986 Jul |
Date Detail:
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Created Date: 1986-08-21 Completed Date: 1986-08-21 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: F74-80 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acute Disease Amino Acids / metabolism* Aminoisobutyric Acids / metabolism Animals Biological Transport, Active / drug effects Dose-Response Relationship, Drug Extracellular Space / analysis, drug effects Insulin / pharmacology* Kidney Failure, Acute / metabolism Male Muscles / drug effects, metabolism* Rats Rats, Inbred Strains Time Factors Uremia / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AM-26903/AM/NIADDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Aminoisobutyric Acids; 11061-68-0/Insulin; 2566-34-9/2-(methylamino)isobutyric acid; 62-57-7/2-aminoisobutyric acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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