Document Detail


Syringotoxin pore formation and inactivation in human red blood cell and model bilayer lipid membranes.
MedLine Citation:
PMID:  12488047     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of syringotoxin (ST), a member of the cyclic lipodepsipeptides family (CLPs) produced by Pseudomonas syringae pv. syringae on the membrane permeability of human red blood cells (RBCs) and model bilayer lipid membranes (BLMs) was studied and compared to that of two recently investigated CLPs, syringomycin E (SRE) and syringopeptin 22A (SP22A) [Biochim. Biophys. Acta 1466 (2000) 79 and Bioelectrochemistry 52 (2000) 161]. The permeability-increasing effect of ST on RBCs was the least among the three CLPs. A time-dependent ST pore inactivation was observed on RBCs at 20 and 37 degrees C but not at 8 degrees C. From the kinetic model worked out parameters as permeability coefficient of RBC membrane for 86Rb(+) and pores mean lifetime were calculated. A shorter pores mean lifetime was calculated at 37 degrees C then at 20 degrees C, which gave us an explanation for the unusual slower rate of tracer efflux measured at 37 degrees C then that at 20 degrees C. The results obtained on BLM showed that the pore inactivation was due to a decrease in the number of pores but not to a change of their dwell time or conductance.
Authors:
Zsófia Szabó; Pál Gróf; Ludmila V Schagina; Philip A Gurnev; Jon Y Takemoto; Edit Mátyus; Katalin Blaskó
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1567     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2002 Dec 
Date Detail:
Created Date:  2002-12-18     Completed Date:  2003-02-12     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  143-9     Citation Subset:  IM    
Affiliation:
Institute of Biophysics and Radiation Biology, Semmelweis University Budapest, Bupapest VIII, Puskin u. POB 263, H-1444 Budapest, Hungary.
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MeSH Terms
Descriptor/Qualifier:
Cell Membrane Permeability / drug effects*
Erythrocytes / drug effects*,  metabolism
Hemoglobins / metabolism
Humans
Lipid Bilayers*
Peptides, Cyclic / pharmacology*
Protein Transport
Chemical
Reg. No./Substance:
0/Hemoglobins; 0/Lipid Bilayers; 0/Peptides, Cyclic; 65547-14-0/syringotoxin B

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