| Syringolin A, a new plant elicitor from the phytopathogenic bacterium Pseudomonas syringae pv. syringae, inhibits the proliferation of neuroblastoma and ovarian cancer cells and induces apoptosis. | |
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MedLine Citation:
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PMID: 17109642 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Syringolin A is a new plant elicitor produced by the plant pathogen Pseudomonas syringae pv. syringae. The goal of this study was to investigate whether syringolin A exhibits anti-proliferative properties in cancer cells. The treatment of human neuroblastoma (NB) cells (SK-N-SH and LAN-1) and human ovarian cancer cells (SKOV3) with syringolin A (0-100 microm) inhibited cell proliferation in a dose-dependent manner. The IC(50) (50% inhibition) for each cell line ranged between 20 microm and 25 microm. In SK-N-SH cells, the treatment with 20 microm syringolin A led to a rapid (24 h) increase of the apoptosis-associated tumour suppressor protein p53. In addition, we found that the treatment of SK-N-SH cells caused severe morphological changes after 48 h such as rounding of cells and loss of adherence, both conditions observed during apoptosis. The induction of apoptosis by syringolin A was confirmed by both poly (ADP-ribose) polymerase (PARP) cleavage and annexin V assay. Taken together, we show for the first time that the natural product syringolin A exhibits anti-proliferative activity and induces apoptosis. Syringolin A and structurally modified syringolin A derivatives may serve as new lead compounds for the development of novel anticancer drugs. |
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Authors:
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C S Coleman; J P Rocetes; D J Park; C J Wallick; B J Warn-Cramer; K Michel; R Dudler; A S Bachmann |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cell proliferation Volume: 39 ISSN: 0960-7722 ISO Abbreviation: Cell Prolif. Publication Date: 2006 Dec |
Date Detail:
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Created Date: 2006-11-19 Completed Date: 2007-01-04 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 9105195 Medline TA: Cell Prolif Country: England |
Other Details:
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Languages: eng Pagination: 599-609 Citation Subset: IM |
Affiliation:
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Cancer Research Center of Hawaii, University of Hawaii at Manoa, Honolulu, HI 96813, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / chemistry, pharmacology* Apoptosis / drug effects* Caspase 3 / metabolism Cell Division / drug effects Cell Line, Transformed Cell Line, Tumor Dose-Response Relationship, Drug Female Fibroblasts / cytology Humans Nervous System Neoplasms / pathology* Neuroblastoma / pathology* Ovarian Neoplasms / pathology* Peptides, Cyclic / chemistry, pharmacology* Poly(ADP-ribose) Polymerases / metabolism Proto-Oncogene Proteins c-akt / metabolism Pseudomonas syringae / chemistry Rats Tumor Suppressor Protein p53 / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Peptides, Cyclic; 0/Tumor Suppressor Protein p53; 0/syringolin A; EC 2.4.2.30/Poly(ADP-ribose) Polymerases; EC 2.7.11.1/Proto-Oncogene Proteins c-akt; EC 3.4.22.-/Caspase 3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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