Document Detail


The Syrian Hamster Embryo (SHE) Low pH Cell Transformation Assay.
MedLine Citation:
PMID:  23045007     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The Syrian hamster embryonic (SHE) cell transformation assay (CTA) at pH 6.7 can determine the ability of a test article to induce morphological transformation (MT) in cultured SHE cells. The assay uses SHE cells prepared at gestation day ∼13 and frozen. Target cells are seeded onto a layer of feeder cells (X-ray irradiated SHE cells) and treated with test article for 24 hr or 7 days. After a growth period of 7 days, the cells are fixed, stained, and evaluated for MT. Normal colonies typically contain a monolayer of cells with an organized, often flowing, pattern of growth and minimal cell criss-crossing at a confluent density. Transformed colonies contain randomly oriented, stacked cells, with cell criss-crossing throughout the colony. The transformed cells are frequently more basophilic than their normal counterparts, with increased nuclear/cytoplasmic ratios. This assay provides a valuable tool for evaluating the carcinogenic potential of a test article. Curr. Protoc. Toxicol. 35:20.3.1-20.3.16. © 2008 by John Wiley & Sons, Inc.
Authors:
Kamala Pant; Marilyn J Aardema
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Current protocols in toxicology / editorial board, Mahin D. Maines (editor-in-chief) ... [et al.]     Volume:  Chapter 20     ISSN:  1934-9262     ISO Abbreviation:  Curr Protoc Toxicol     Publication Date:  2008 Feb 
Date Detail:
Created Date:  2012-10-09     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9816330     Medline TA:  Curr Protoc Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  Unit20.3     Citation Subset:  IM    
Affiliation:
BioReliance, Rockville, Maryland.
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