| Syrene biosynthesis from glucose by engineered E. coli. | |
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MedLine Citation:
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PMID: 21722749 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized l-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an l-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as ∼300mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks. |
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Authors:
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Rebekah McKenna; David R Nielsen |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-6-23 |
Journal Detail:
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Title: Metabolic engineering Volume: - ISSN: 1096-7184 ISO Abbreviation: - Publication Date: 2011 Jun |
Date Detail:
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Created Date: 2011-7-4 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815657 Medline TA: Metab Eng Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011 Elsevier Inc. All rights reserved. |
Affiliation:
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Chemical Engineering, Arizona State University, 501 E. Tyler Mall, ECG 301, Tempe, AZ 85287-6106, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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