Document Detail

Synthetic pyrethroid cypermethrin induced cellular damage in reproductive tissues of Drosophila melanogaster: Hsp70 as a marker of cellular damage.
MedLine Citation:
PMID:  16418893     Owner:  NLM     Status:  MEDLINE    
We tested a working hypothesis of whether the synthetic pyrethroid cypermethrin, used worldwide for insecticidal purpose, causes adverse effects on reproduction in Drosophila melanogaster. Freshly eclosed first instar larvae of a transgenic strain of Drosophila melanogaster, Bg9, transgenic for hsp70 (hsp70-lacZ), were transferred to different dietary concentrations of the test chemical (0.002, 0.02, 0.2, 0.5, and 50.0 ppm). Larval mortality was observed at the higher dosed groups (0.2, 0.5, and 50.0 ppm). Following pair mating of virgin flies emerging from the treatment groups, a significant (p<0.05) effect on reproduction was observed in the lowest two dietary concentrations of the test chemical as compared to control. The test chemical exhibited a hazardous effect on the reproductive organs of the exposed organism as evident by Hsp70 expression and tissue damage. The impact of damage was comparatively more prominent in male flies than in females. Hsp70 expression was restricted only within the testis lobes of male, while ovary in the female fly did not exhibit any Hsp70 expression. Interestingly, the accessory glands of male flies in these treatment groups reflected intense tissue damage as evident by Trypan Blue staining. This was further corroborated by ultrastructural changes like higher vacuolization and disorganized filamentous bodies in the accessory glands of these groups. The present study indicates a profound effect on reproduction by cypermethrin and suggests the protective role of hsp70.
Indranil Mukhopadhyay; Hifzur Rahman Siddique; Virendra Kumar Bajpai; Daya Krishna Saxena; Debapratim Kar Chowdhuri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-01-13
Journal Detail:
Title:  Archives of environmental contamination and toxicology     Volume:  51     ISSN:  0090-4341     ISO Abbreviation:  Arch. Environ. Contam. Toxicol.     Publication Date:  2006 Nov 
Date Detail:
Created Date:  2006-10-16     Completed Date:  2006-12-13     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0357245     Medline TA:  Arch Environ Contam Toxicol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  673-80     Citation Subset:  IM    
Embryotoxicology Section, Industrial Toxicology Research Centre, P.O. Box No. 80, M.G. Marg, Lucknow, 226 001, Uttar Pradesh, India.
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MeSH Terms
Biological Markers
Drosophila melanogaster
Genitalia, Female / drug effects*,  pathology
Genitalia, Male / drug effects*,  pathology
HSP70 Heat-Shock Proteins / analysis*,  physiology
Insecticides / toxicity*
Microscopy, Electron
Pyrethrins / toxicity*
Reg. No./Substance:
0/Biological Markers; 0/HSP70 Heat-Shock Proteins; 0/Insecticides; 0/Pyrethrins; 52315-07-8/cypermethrin

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