Document Detail

Synthetic peptide (P-21) derived from Asp-hemolysin inhibits the induction of apoptosis on HUVECs by lysophosphatidylcholine.
MedLine Citation:
PMID:  16651717     Owner:  NLM     Status:  MEDLINE    
Lysophosphatidylcholine (LPC), formed during low-density lipoprotein (LDL) oxidation and located within atherosclerotic plaques, regulates a variety of cellular functions, some of which could be construed to promote atherosclerotic lesion development, including vascular muscle cell proliferation, monocyte attraction, and endothelial cell apoptosis. We have previously reported that the synthetic peptide derived from Asp-hemolysin, named P-21, inhibits oxidized LDL (OxLDL)-induced macrophage proliferation through binding of P-21 to OxLDL. In this study, to clarify the interaction between P-21 and LPC as a typical lipid moiety of OxLDL, we examined the influence of P-21 on LPC-induced apoptosis in human umbilical vein endothelial cells (HUVECs). Based on flow cytometric analysis, using annexin V-fluorescein isothiocyanate and propidium iodide as probes to assess apoptosis, LPC induced the apoptosis of HUVECs, and P-21 significantly inhibited this activity by 82.4%. Furthermore, dissociation-enhanced lanthanide fluorometric immunoassay indicated that LPC inhibited the binding of P-21 to OxLDL in a dose-dependent manner. A 50% inhibition dose was estimated to be 4.65 microM of LPC. These results suggest that P-21 inhibits LPC-induced HUVEC apoptosis through binding of P-21 to LPC.
Hiromu Tsutsumi; Takeshi Kumagai; Saori Naitoo; Keiichi Ebina; Katsushi Yokota
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Biological & pharmaceutical bulletin     Volume:  29     ISSN:  0918-6158     ISO Abbreviation:  Biol. Pharm. Bull.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-02     Completed Date:  2006-07-11     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9311984     Medline TA:  Biol Pharm Bull     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  907-10     Citation Subset:  IM    
First Department of Hygienic Chemistry, Tohoku Pharmaceutical University, Sendai, Miyagi, Japan.
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MeSH Terms
Annexin A5 / metabolism
Apoptosis / drug effects*
Cell Survival / drug effects
Cells, Cultured
Endothelial Cells / drug effects
Flow Cytometry
Lipoproteins, LDL / chemistry
Lysophosphatidylcholines / antagonists & inhibitors*,  metabolism,  pharmacology*
Protein Binding
Reg. No./Substance:
0/Annexin A5; 0/Lipoproteins, LDL; 0/Lysophosphatidylcholines

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