Document Detail


Synthetic and endogenous testosterone conjugates: acid hydrolysis studied by radioimmunoassay.
MedLine Citation:
PMID:  668115     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The acid hydrolysis of synthetic testosterone sulphate and glucuronide by sulphuric acid has been studied by measuring the release of uncojugated testosterone by radioimmunoassay. The sulphate was hydrolyzed at lower concentration of acid but no conditions were found where only the sulphate was completely hydrolyzed without any hydrolysis of the glucuronide. Results of similar studies on human urine, however, suggested that human urine contains three conjugates of testosterone and 5 alpha-dihydrotestosterone: the sulphate and glucuronide and also a very acid labile conjugate hitherto unknown. Hydrochloric acid has stronger hydrolyzing effect at the same normality and released more unconjugated testosterone in urine at the same pH than did sulphuric acid. A complete hydrolysis of urinary conjugates in 3 molar sulphuric acid was accomplished without destruction of immunoassayable testosterone and constitutes a suitable method for the determination of the total testosterone content in urine. The method was compared with other hydrolyzing methods by measuring the testosterone release from urinary conjugates after their extraction and separation by by thin-layer chromatography.
Authors:
J M Kjeld; C M Puah; G F Joplin
Related Documents :
2453525 - Simultaneous determination of the major acidic metabolites of catecholamines and seroto...
1842595 - Determining of actual activities of acid and alkaline ribonuclease in human serum and u...
2397085 - Biosynthesis of salsolinol, a tetrahydroisoquinoline alkaloid, in healthy subjects.
837525 - Effect of structure on the excretion of steroidal acids in human urine and their potent...
21246485 - Gastroprotective effect and cytotoxicity of carnosic acid derivatives.
18178005 - Comparison of different advanced oxidation processes (aops) in the presence of perovski...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Clinica chimica acta; international journal of clinical chemistry     Volume:  86     ISSN:  0009-8981     ISO Abbreviation:  Clin. Chim. Acta     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-09-15     Completed Date:  1978-09-15     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  1302422     Medline TA:  Clin Chim Acta     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  235-43     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Chromatography, Thin Layer
Glucuronidase
Humans
Hydrochloric Acid
Hydrolysis
Male
Radioimmunoassay
Sulfuric Acids
Testosterone / urine*
Chemical
Reg. No./Substance:
0/Sulfuric Acids; 58-22-0/Testosterone; 7647-01-0/Hydrochloric Acid; EC 3.2.1.31/Glucuronidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  A micromethod for separation and identification of digestive enzymes in brush border membrane fragme...
Next Document:  Effect of cycloleucine on amino acid accumulation by human diploid fibroblasts.