| Synthetic dimyristoylphosphatidylcholine liposomes assimilating into high-density lipoprotein promote regression of atherosclerotic lesions in cholesterol-fed rabbits. | |
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MedLine Citation:
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PMID: 20876082 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have reported recently that enrichment of high-density lipoprotein (HDL) with phosphatidylcholine (PC) liposomes is effective in solubilizing cholesterol from isolated human atherosclerotic plaques. In the present study, we investigated the in vivo effect of enrichment of HDL with PC on regression of diet-induced atherosclerosis in rabbits. As part of the study, a preliminary in vitro study on blood collected from the cholesterol-fed rabbits was performed to assess the capacity of the HDL density (d > 1.063 g/mL) plasma fraction from cholesterol-fed rabbits to assimilate multilamellar liposomes of synthetic dimyristoylphosphatidylcholine (DMPC). This was compared with the capacities of egg- and soy-PC liposomes to be assimilated into the HDL density plasma fraction. The capacity of the HDL density fraction to absorb PC from DMPC liposomes (11.5 mg/mL) was more than 10 times greater than egg or soy liposomes. Therefore, DMPC liposomes were chosen to infuse into cholesterol-fed rabbits. Cholesterol-fed rabbits infused weekly with DMPC liposomes (300 mg/kg body weight) for five weeks had significantly decreased aortic cholesterol contents (P < 0.05) compared with saline-infused cholesterol-fed controls. Atherosclerotic plaque volume, as measured by a type of new magnetic resonance imaging analysis, also decreased significantly (P < 0.05) after DMPC treatment. The present findings suggest that the enrichment of HDL with PC via intravenous infusion of synthetic DMPC liposomes could be a potential therapeutic approach for atherosclerotic plaque regression. |
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Authors:
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Byung H Simon Cho; Jeong-Ro Park; Manabu T Nakamura; Boris M Odintsov; Matthew A Wallig; Byung-Hong Chung |
Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-27 |
Journal Detail:
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Title: Experimental biology and medicine (Maywood, N.J.) Volume: 235 ISSN: 1535-3699 ISO Abbreviation: Exp. Biol. Med. (Maywood) Publication Date: 2010 Oct |
Date Detail:
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Created Date: 2010-09-30 Completed Date: 2010-10-28 Revised Date: 2011-03-11 |
Medline Journal Info:
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Nlm Unique ID: 100973463 Medline TA: Exp Biol Med (Maywood) Country: England |
Other Details:
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Languages: eng Pagination: 1194-203 Citation Subset: IM |
Affiliation:
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Harlan E Moore Heart Research Foundation, Champaign, IL 61820, USA. bhcho@illinois.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Aorta / pathology Atherosclerosis / blood*, drug therapy*, pathology Cholesterol / blood Cholesterol, Dietary / administration & dosage Dimyristoylphosphatidylcholine / administration & dosage* Infusions, Intravenous Lipoproteins, HDL / blood* Liposomes / administration & dosage Male Rabbits |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol, Dietary; 0/Lipoproteins, HDL; 0/Liposomes; 13699-48-4/Dimyristoylphosphatidylcholine; 57-88-5/Cholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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