| Synthetic copolymer kit for radionuclide blood-pool imaging. | |
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MedLine Citation:
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PMID: 7965173 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A synthetic blood pool imaging agent labeled with 99mTc is reported. METHODS: The agent, methoxypolyethylene glycol-poly-L-lysyl-diethylenetriaminepentaacetate monoamide was synthesized from a covalent graft copolymer of methoxypolyethylene glycol succinate (molecular weight 5.1 kD) and poly-L-lysine (molecular weight average 35.6 kD) with subsequent modification of the product with diethylenetriamineacetyl residues. The polymer was formulated into a kit that contained Sn(II) and sodium acetate for radiolabeling with 99mTc. Biodistribution studies were performed in rats. Blood-pool imaging and blood clearance determination was carried out in rabbits and in a rhesus monkey. RESULTS: The 99mTc-labeled agent [specific activity greater than 3.7 GBq/mg; radiochemical purity more than 98% by thin-layer and high-performance liquid chromatography (HPLC)] demonstrated remarkable stability in solution (pH 5.5-6.5) with no radioactive products of degradation detectable by HPLC even at 24 hr postlabeling. The agent exhibited prolonged circulation in the blood with a half-life of 31.5 hr in rabbits. Biodistribution in rats showed a lack of substantial accumulation of the agent in the reticuloendothelial system. Sequential acquisitions were performed in a rhesus monkey. The 99mTc-labeled polymer kit was compared with the 99mTc-red blood cells (RBCs) labeled in vitro. Both methods produced similar heart-to-lung ratios. The ratios remained essentially unchanged for up to 15 hr postinjection. CONCLUSION: The 99mTc-labeled methoxypolyethylene glycol-poly-L-lysyl-diethylenetriamine pentaacetate monoamide is an attractive alternative to radiolabeled RBCs for blood pool imaging applications. |
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Authors:
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A A Bogdanov; R J Callahan; R A Wilkinson; C Martin; J A Cameron; A J Fischman; T J Brady; R Weissleder |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Journal of nuclear medicine : official publication, Society of Nuclear Medicine Volume: 35 ISSN: 0161-5505 ISO Abbreviation: J. Nucl. Med. Publication Date: 1994 Nov |
Date Detail:
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Created Date: 1994-12-12 Completed Date: 1994-12-12 Revised Date: 2004-11-17 |
Medline Journal Info:
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Nlm Unique ID: 0217410 Medline TA: J Nucl Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1880-6 Citation Subset: IM |
Affiliation:
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Department of Radiology, Massachusetts General Hospital, Boston. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Gated Blood-Pool Imaging* Humans Macaca mulatta Male Rabbits Rats Rats, Sprague-Dawley Reagent Kits, Diagnostic* Technetium Tc 99m Pentetate / analogs & derivatives*, diagnostic use Tissue Distribution |
| Chemical | |
Reg. No./Substance:
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0/Reagent Kits, Diagnostic; 0/technetium Tc 99m methoxypolyethylene glycol-polylysyl-DTPA monoamide; 65454-61-7/Technetium Tc 99m Pentetate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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