Document Detail


Synthetic capacity of Arabidopsis phosphatidylinositol synthase 1 expressed in Escherichia coli.
MedLine Citation:
PMID:  14563413     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Phosphatidylinositol (PtdIns) synthase 1 from the plant Arabidopsis thaliana has been expressed in Escherichia coli in order to study the synthetic capacities of the enzyme. Analysis of the total fatty acid content of the bacteria shows that PtdIns synthase activity does not have a profound effect on the proportions of the different fatty acids produced, even if the presence of an extra acidic phospholipid leads to a global reduction of the lipid content. A closer analysis carried out on individual phospholipids reveals a global fatty acid composition almost unchanged in the two major bacterial lipids phosphatidylethanolamine (PtdEtn) and phosphatidylglycerol (PtdGro). Phosphatidylinositol has a very unusual composition that shows the ability of the plant enzyme to use CDP-diacylglycerol molecular species absent from plants. We identified the various PtdIns molecular species. They represent a pool of the major molecular species of PtdEtn and PtdGro. These results, together with the determination of the apparent affinity constants of AtPIS1 for myo-inositol and CDP-diacylglycerol, allow us to discuss some of the constraints of PtdIns synthesis in plants in terms of specificity, which will depend on the subcellular localization of the protein.
Authors:
Anne-Marie Justin; Jean-Claude Kader; Sylvie Collin
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochimica et biophysica acta     Volume:  1634     ISSN:  0006-3002     ISO Abbreviation:  Biochim. Biophys. Acta     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-17     Completed Date:  2003-12-04     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0217513     Medline TA:  Biochim Biophys Acta     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  52-60     Citation Subset:  IM    
Affiliation:
Université Pierre et Marie Curie, UMR 7632 CNRS/Paris 6, Tour 53, Case Courrier 154, 4, Place Jussieu, 75252 Cedex 05, Paris, France.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / enzymology*
Arabidopsis Proteins
CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase
Escherichia coli / genetics,  metabolism*
Fatty Acids / metabolism
Lipid Metabolism
Lipids / chemistry
Membrane Proteins
Phosphatidylinositols / metabolism
Plant Proteins / genetics,  metabolism*
Transferases (Other Substituted Phosphate Groups) / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/Arabidopsis Proteins; 0/Fatty Acids; 0/Lipids; 0/Membrane Proteins; 0/Phosphatidylinositols; 0/Plant Proteins; EC 2.7.8.-/Transferases (Other Substituted Phosphate Groups); EC 2.7.8.11/ATPIS1 protein, Arabidopsis; EC 2.7.8.11/CDP-Diacylglycerol-Inositol 3-Phosphatidyltransferase

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