Document Detail


Synthetic activities of mass cultures and clones of human gingival fibroblasts.
MedLine Citation:
PMID:  3943601     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The percentage of synthesis dedicated to collagen is elevated in low-density cultures of human gingival fibroblasts, as is per-cell total protein synthetic activity and glycosaminoglycan accumulation. These observations can be explained, in part, by a decrease in membrane transport of precursor substance in high-density cultures. Synthetic activity by human fibroblasts can be reliably assayed in vitro using as few as 500 cells sparsely seeded. Such low-cell number assay is essential for study of single-cell clones, where replicative life span is limited.
Authors:
T M Hassell; D V Provenza; R A Foster
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Experientia     Volume:  42     ISSN:  0014-4754     ISO Abbreviation:  Experientia     Publication Date:  1986 Jan 
Date Detail:
Created Date:  1986-03-06     Completed Date:  1986-03-06     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0376547     Medline TA:  Experientia     Country:  SWITZERLAND    
Other Details:
Languages:  eng     Pagination:  66-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Biological Transport
Cell Count
Cell Division
Cell Membrane / metabolism
Cell Survival
Cells, Cultured
Clone Cells / cytology,  metabolism
Collagen / biosynthesis*
Fibroblasts / cytology,  metabolism
Gingiva / cytology*,  metabolism
Glycosaminoglycans / biosynthesis*
Humans
Interphase
Proline / metabolism
Protein Biosynthesis*
Grant Support
ID/Acronym/Agency:
DE-00123/DE/NIDCR NIH HHS; DE-05459/DE/NIDCR NIH HHS; DE-06671/DE/NIDCR NIH HHS
Chemical
Reg. No./Substance:
0/Glycosaminoglycans; 147-85-3/Proline; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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