| Synthetic DNA immunogen encoding hepatitis B core antigen drives immune response in liver. | |
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MedLine Citation:
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PMID: 23037809 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The prevalence of hepatitis B virus (HBV) infection in Asia and sub-Sahara Africa is alarming. With quarter of a billion people chronically infected worldwide and at risk of developing liver cancer, the need for a prophylactic or therapeutic vaccination approach that can effectively induce protective responses against the different genotypes of HBV is more important than ever. Such a strategy will require both the induction of a strong antigen-specific immune response and the subsequent deployment of immune response towards the liver. Here, we assessed the ability of a synthetic DNA vaccine encoding a recombinant consensus plasmid from genotype A through E of the HBV core antigen (HBcAg), to drive immunity in the liver. Intramuscular vaccination induced both strong antigen-specific T cell and high titer antibody responses systematically and in the liver. Furthermore, immunized mice showed strong cytotoxic responses that eliminate adoptively transferred HBV-coated target cells. Importantly, vaccine-induced immune responses provided protection from HBcAg plasmid-based liver transfection in a hydrodynamic liver transfection model. These data provide important insight into the generation of peripheral immune responses that are recruited to the liver-an approach that can be beneficial in the search for vaccines or immune-therapies to liver disease. |
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Authors:
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N Obeng-Adjei; D K Choo; J Saini; J Yan; P Pankhong; A Parikh; J S Chu; D B Weiner |
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Publication Detail:
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Type: Journal Article Date: 2012-10-05 |
Journal Detail:
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Title: Cancer gene therapy Volume: 19 ISSN: 1476-5500 ISO Abbreviation: Cancer Gene Ther. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-10-18 Completed Date: 2013-03-11 Revised Date: 2013-04-30 |
Medline Journal Info:
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Nlm Unique ID: 9432230 Medline TA: Cancer Gene Ther Country: England |
Other Details:
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Languages: eng Pagination: 779-87 Citation Subset: IM |
Affiliation:
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Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Administration, Intranasal Animals Antibody Formation CD8-Positive T-Lymphocytes / immunology, virology DNA, Viral / administration & dosage, genetics, immunology* Enzyme-Linked Immunospot Assay Female Genes, Viral Genotype Hepatitis B / immunology, therapy Hepatitis B Core Antigens / administration & dosage, genetics, immunology* Hepatitis B Vaccines / administration & dosage, genetics, immunology* Hepatitis B virus / genetics, immunology Hepatocytes / immunology, virology Liver / immunology*, virology Mice Mice, Inbred BALB C Plasmids / metabolism Transfection Vaccination / methods Vaccines, DNA / administration & dosage, immunology* |
| Grant Support | |
ID/Acronym/Agency:
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P01 AI071739/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Viral; 0/Hepatitis B Core Antigens; 0/Hepatitis B Vaccines; 0/Vaccines, DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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