Document Detail


Synthetic DNA immunogen encoding hepatitis B core antigen drives immune response in liver.
MedLine Citation:
PMID:  23037809     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The prevalence of hepatitis B virus (HBV) infection in Asia and sub-Sahara Africa is alarming. With quarter of a billion people chronically infected worldwide and at risk of developing liver cancer, the need for a prophylactic or therapeutic vaccination approach that can effectively induce protective responses against the different genotypes of HBV is more important than ever. Such a strategy will require both the induction of a strong antigen-specific immune response and the subsequent deployment of immune response towards the liver. Here, we assessed the ability of a synthetic DNA vaccine encoding a recombinant consensus plasmid from genotype A through E of the HBV core antigen (HBcAg), to drive immunity in the liver. Intramuscular vaccination induced both strong antigen-specific T cell and high titer antibody responses systematically and in the liver. Furthermore, immunized mice showed strong cytotoxic responses that eliminate adoptively transferred HBV-coated target cells. Importantly, vaccine-induced immune responses provided protection from HBcAg plasmid-based liver transfection in a hydrodynamic liver transfection model. These data provide important insight into the generation of peripheral immune responses that are recruited to the liver-an approach that can be beneficial in the search for vaccines or immune-therapies to liver disease.
Authors:
N Obeng-Adjei; D K Choo; J Saini; J Yan; P Pankhong; A Parikh; J S Chu; D B Weiner
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Publication Detail:
Type:  Journal Article     Date:  2012-10-05
Journal Detail:
Title:  Cancer gene therapy     Volume:  19     ISSN:  1476-5500     ISO Abbreviation:  Cancer Gene Ther.     Publication Date:  2012 Nov 
Date Detail:
Created Date:  2012-10-18     Completed Date:  2013-03-11     Revised Date:  2013-08-06    
Medline Journal Info:
Nlm Unique ID:  9432230     Medline TA:  Cancer Gene Ther     Country:  England    
Other Details:
Languages:  eng     Pagination:  779-87     Citation Subset:  IM    
Affiliation:
Department of Pathology and Laboratory Medicine, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
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MeSH Terms
Descriptor/Qualifier:
Administration, Intranasal
Animals
Antibody Formation
CD8-Positive T-Lymphocytes / immunology,  virology
DNA, Viral / administration & dosage,  genetics,  immunology*
Enzyme-Linked Immunospot Assay
Female
Genes, Viral
Genotype
Hepatitis B / immunology,  therapy
Hepatitis B Core Antigens / administration & dosage,  genetics,  immunology*
Hepatitis B Vaccines / administration & dosage,  genetics,  immunology*
Hepatitis B virus / genetics,  immunology
Hepatocytes / immunology,  virology
Liver / immunology*,  virology
Mice
Mice, Inbred BALB C
Plasmids / metabolism
Transfection
Vaccination / methods
Vaccines, DNA / administration & dosage,  immunology*
Grant Support
ID/Acronym/Agency:
P01 AI071739/AI/NIAID NIH HHS; R01 AI092843/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Viral; 0/Hepatitis B Core Antigens; 0/Hepatitis B Vaccines; 0/Vaccines, DNA
Comments/Corrections

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