Document Detail


Synthetase polyspecificity as a tool to modulate protein function.
MedLine Citation:
PMID:  22041062     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The site-specific incorporation of unnatural amino acids (UAAs) into proteins in bacteria is made possible by the evolution of aminoacyl-tRNA synthetases that selectively recognize and aminoacylate the amino acid of interest. Recently we have discovered that some of the previously evolved aaRSs display a degree of polyspecificity and are capable of recognizing multiple UAAs. Herein we report the polyspecificity of an aaRS evolved to encode a comarin containing amino acid. This polyspecificity was then exploited to introduce several UAAs into the fluorophore of GFP, altering its photophysical properties.
Authors:
Douglas D Young; Steffen Jockush; Nicholas J Turro; Peter G Schultz
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2011-10-08
Journal Detail:
Title:  Bioorganic & medicinal chemistry letters     Volume:  21     ISSN:  1464-3405     ISO Abbreviation:  Bioorg. Med. Chem. Lett.     Publication Date:  2011 Dec 
Date Detail:
Created Date:  2011-11-21     Completed Date:  2012-03-12     Revised Date:  2013-10-17    
Medline Journal Info:
Nlm Unique ID:  9107377     Medline TA:  Bioorg Med Chem Lett     Country:  England    
Other Details:
Languages:  eng     Pagination:  7502-4     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
Affiliation:
Department of Chemistry, The Scripps Research Institute, 10550 N. Torrey Pines Rd., La Jolla, CA 92037, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Amino Acids / metabolism*
Amino Acyl-tRNA Synthetases / metabolism*
Bacteria / metabolism
Green Fluorescent Proteins / genetics,  metabolism
Substrate Specificity
Grant Support
ID/Acronym/Agency:
F32 CA144213/CA/NCI NIH HHS; F32CA144213/CA/NCI NIH HHS
Chemical
Reg. No./Substance:
0/Amino Acids; 147336-22-9/Green Fluorescent Proteins; EC 6.1.1.-/Amino Acyl-tRNA Synthetases
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Synthesis of locked pyranosyl nucleic acid (LpNA).
Next Document:  Carbamates of 4'-demethyl-4-deoxypodophyllotoxin: Synthesis, cytotoxicity and cell cycle effects.