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Synthesized Pheophorbide a-mediated photodynamic therapy induced apoptosis and autophagy in human oral squamous carcinoma cells.
MedLine Citation:
PMID:  22742535     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
J Oral Pathol Med (2012) Background:  Pheophorbide a (Pa) is a chlorine-based photosensitizer derived from an ethnopharmacological herb, and our group recently synthesized Pa by the removal of a magnesium ion and a phytyl group from chlorophyll-a. In this study, the effect of photodynamic therapy (PDT) with synthesized Pa was examined in a human oral squamous cell carcinoma (OSCC) cells. Methods:  Cells were treated with PDT with Pa, and reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨ (m)) were examined. Apoptosis was measured using annexin V staining and immunoblot. Autophagy was characterized by the increase in LC3B-II and the formation of autophagosome and acidic vesicular organelles (AVOs). Results:  Pa-PDT inhibited the proliferation of OSCC cells in a dose-dependent manner. Pa-PDT increased the number of apoptotic cells by inactivating ERK pathway. Pa-PDT also induced autophagy in OSCC cells evidenced by the increased levels of LC3 type II expression and the accumulation of AVOs. The inhibition of autophagy enhanced Pa-PDT-mediated cytotoxicity through an increase in necrosis. Conclusions:  These results suggest that synthesized Pa-PDT exerts anti-tumor effects by inducing apoptosis and autophagy and provide novel evidence that Pa-PDT induces autophagy, and autophagy inhibition enhances Pa-PDT-mediated necrosis in OSCC cells.
Authors:
Mee Young Ahn; Hyo-Eun Yoon; Seong-Min Kwon; Jun Lee; Seung-Ki Min; Yong-Chul Kim; Sang-Gun Ahn; Jung-Hoon Yoon
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-6-28
Journal Detail:
Title:  Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology     Volume:  -     ISSN:  1600-0714     ISO Abbreviation:  -     Publication Date:  2012 Jun 
Date Detail:
Created Date:  2012-6-29     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8911934     Medline TA:  J Oral Pathol Med     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© 2012 John Wiley & Sons A/S.
Affiliation:
Department of Pathology, Research Center for Oral disease Regulation of the Aged, School of Dentistry, Chosun University, Gwangju, Korea Department of Oral & Maxillofacial Surgery, College of Dentistry, Daejeon Dental Hospital, Wonkwang Bone Regeneration Institute, Wonkwang University, Daejeon, Korea Department of Life Science, Gwangju Institute of Science and Technology, Gwangju, Korea Department of Oral & Maxillofacial Pathology, College of Dentistry, Daejeon Dental Hospital, Wonkwang Bone Regeneration Institute, Wonkwang University, Daejeon, Korea.
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