Document Detail


Synthesis of vitamin B12 by various species of mycobacteria.
MedLine Citation:
PMID:  345687     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The synthesis of Vitamin B12 by five reference strains of species of mycobacteria, the influence of cobalt upton the synthesis and the correlation between the phase of growth and quantitative biosynthesis of Vitamin B12 were studied. Assays were performed on cell extracts and culture filtrates using Lactobacillus leichmannii ATCC7830 (tube method). All five species synthesized Vitamin B12, which could be deomonstrated both in cell extracts and in the culture filtrates. An optimum accumulation of Vitamin B12 by BCG and M. phlei could be observed on the 30th day of cultivation. Addition of cobalt did not influence the growth of BCG and M. phlei, but markedly enhanced the production of Vitamin B12. Cells contained much more Vitamin B12 than culture filtrates did. Vitamin B12 levels in M. smegmatis, M. fortuitum and M. asiaticum were lower than those in BCG and M. phlei.
Authors:
V Karasseva; J G Weiszfeiler; Z Lengyel
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Erste Abteilung Originale. Reihe A: Medizinische Mikrobiologie und Parasitologie     Volume:  239     ISSN:  0300-9688     ISO Abbreviation:  Zentralbl Bakteriol Orig A     Publication Date:  1977 Dec 
Date Detail:
Created Date:  1978-05-20     Completed Date:  1978-05-20     Revised Date:  2009-06-05    
Medline Journal Info:
Nlm Unique ID:  0331570     Medline TA:  Zentralbl Bakteriol Orig A     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  514-20     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
BCG Vaccine
Culture Media
Mycobacterium / metabolism*
Mycobacterium bovis / metabolism
Mycobacterium phlei / metabolism
Time Factors
Vitamin B 12 / biosynthesis*
Chemical
Reg. No./Substance:
0/BCG Vaccine; 0/Culture Media; 68-19-9/Vitamin B 12

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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