Document Detail


Synthesis and utilization of GDP-D-arabinopyranoside.
MedLine Citation:
PMID:  9025949     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We describe a procedure for the enzymatic synthesis of labeled or unlabeled GDP-D-arabinopyranoside. This method uses two enzymes purified from pig kidney: an L-fucokinase and a GDP-L-fucose pyrophosphorylase. The isolated GDP-D-[3H]arabinose served as a precursor for arabinose addition to lipophosphoglycan (LPG) of Leishmania major, using a parasite membrane fraction as the source of arabinosyltransferase. The procedures described provide a useful means for obtaining radiolabeled GDP-D-arabinopyranoside to study synthesis of D-arabinopyranoside-containing glycoconjugates.
Authors:
S Park; I Pastuszak; B J Mengeling; S J Turco; A D Elbein
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  244     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1997 Jan 
Date Detail:
Created Date:  1997-04-07     Completed Date:  1997-04-07     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  321-7     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biology, University of Arkanasas for Medical Sciences, Little Rock 72211, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Arabinose / analogs & derivatives*
Chromatography, Gel
Crithidia
Guanosine Diphosphate Sugars / chemical synthesis*,  metabolism
Kidney / enzymology
Leishmania major
Nucleotidyltransferases / metabolism
Phosphotransferases (Alcohol Group Acceptor) / metabolism
Sugar Phosphates / chemical synthesis
Swine
Grant Support
ID/Acronym/Agency:
AI-20941/AI/NIAID NIH HHS; DK-21800/DK/NIDDK NIH HHS
Chemical
Reg. No./Substance:
0/Guanosine Diphosphate Sugars; 0/Sugar Phosphates; 0/arabinose-1-phosphate; 130272-39-8/guanidine diphosphate-arabinopyranose; 147-81-9/Arabinose; EC 2.7.1.-/Phosphotransferases (Alcohol Group Acceptor); EC 2.7.1.52/fucokinase; EC 2.7.7.-/Nucleotidyltransferases; EC 2.7.7.30/fucose-1-phosphate guanylyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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