Document Detail

Synthesis and use of fluorescent molecular probes for measuring cell-surface enzymatic oxidation of amino acids and amines in seawater.
MedLine Citation:
PMID:  8317696     Owner:  NLM     Status:  MEDLINE    
A method for investigating cell-surface enzymatic oxidative deamination of amino acids and amines in seawater was developed. This technique used synthetic fluorescent Lucifer Yellow derivatives of the amino acid lysine and the amine cadaverine as molecular probes to investigate oxidation pathways and rates. The probes were chemically stable under the conditions used and did not adsorb to container surfaces. The oxidative deamination of the fluorescent probes added to phytoplankton cultures and the subsequent production of their fluorescent oxidation products could be selectively detected by HPLC at 250 pM levels. This approach allows selective investigation of cell-surface enzymatic oxidation since neither transport of the probes across the cell membrane nor chemical transformation of the probes occurs. Bacteria were also capable of oxidizing the fluorescent amino acid probe.
S Pantoja; C Lee; J F Marecek; B P Palenik
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Analytical biochemistry     Volume:  211     ISSN:  0003-2697     ISO Abbreviation:  Anal. Biochem.     Publication Date:  1993 Jun 
Date Detail:
Created Date:  1993-07-28     Completed Date:  1993-07-28     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0370535     Medline TA:  Anal Biochem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  210-8     Citation Subset:  IM    
Marine Sciences Research Center, State University of New York, Stony Brook 11794-5000.
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MeSH Terms
Algae / enzymology*
Amines / metabolism*
Amino Acid Oxidoreductases / metabolism
Amino Acids / metabolism*,  pharmacokinetics
Amino Acids, Neutral*
Bacteria / metabolism
Cadaverine / metabolism,  pharmacokinetics
Cell Membrane / enzymology,  metabolism
Fluorescent Dyes / chemical synthesis*,  chemistry,  pharmacokinetics
Hydrogen Peroxide / metabolism
Isoquinolines / metabolism,  pharmacokinetics
L-Amino Acid Oxidase
Lysine / metabolism,  pharmacokinetics
Membrane Proteins / metabolism,  pharmacokinetics
Phytoplankton / enzymology,  metabolism
Reg. No./Substance:
0/Amines; 0/Amino Acids; 0/Amino Acids, Neutral; 0/Fluorescent Dyes; 0/Isoquinolines; 0/Membrane Proteins; 462-94-2/Cadaverine; 56-87-1/Lysine; 660-88-8/5-aminovaleric acid; 7722-84-1/Hydrogen Peroxide; 77944-88-8/lucifer yellow; EC 1.4.-/Amino Acid Oxidoreductases; EC Acid Oxidase

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