Document Detail


Synthesis of two mirror image 4-helix junctions derived from glycerol nucleic acid.
MedLine Citation:
PMID:  18407636     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Structural DNA nanotechnology relies on Watson-Crick base pairing rules to assemble DNA motifs into diverse arrangements of geometric shapes and patterns. While substantial effort has been devoted to expanding the programmability of natural DNA, considerably less attention has been given to the development of nucleic acid structures based on non-natural DNA polymers. Here we describe the use of glycerol nucleic acid (GNA), a simple polymer based on an acyclic repeating unit, as an alternative genetic material for assembling nucleic acid nanostructures independent of RNA or DNA recognition. We synthesized two 4-helix junctions based entirely on GNA self-pairing and showed that GNA provides easy access to highly stable nanostructures with left- and right-handed helical configurations.
Authors:
Richard S Zhang; Elizabeth O McCullum; John C Chaput
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-04-12
Journal Detail:
Title:  Journal of the American Chemical Society     Volume:  130     ISSN:  1520-5126     ISO Abbreviation:  J. Am. Chem. Soc.     Publication Date:  2008 May 
Date Detail:
Created Date:  2008-04-30     Completed Date:  2008-06-23     Revised Date:  2008-12-15    
Medline Journal Info:
Nlm Unique ID:  7503056     Medline TA:  J Am Chem Soc     Country:  United States    
Other Details:
Languages:  eng     Pagination:  5846-7     Citation Subset:  IM    
Affiliation:
Center for BioOptical Nanotechnology, Biodesign Institute, and Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287-1604, USA.
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MeSH Terms
Descriptor/Qualifier:
Circular Dichroism
DNA / chemical synthesis,  chemistry
Electrophoresis
Glycerol / analogs & derivatives*,  chemistry
Models, Molecular
Nanostructures / chemistry*
Nucleic Acid Conformation
Nucleic Acids / chemical synthesis*,  chemistry
Oligonucleotides / chemical synthesis*,  chemistry
Organophosphorus Compounds / chemical synthesis,  chemistry
Stereoisomerism
Chemical
Reg. No./Substance:
0/Nucleic Acids; 0/Oligonucleotides; 0/Organophosphorus Compounds; 0/phosphoramidite; 56-81-5/Glycerol; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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