Document Detail


Synthesis and transport of the organic matrix of the spicules in the gorgonian Leptogorgia virgulata (Lamarck) (Coelenterata: Gorgonacea). An autoradiographic investigation.
MedLine Citation:
PMID:  6143616     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The sequence of the synthesis and transport of the organic matrix of spicules has been elucidated in the gorgonian Leptogorgia virgulata by use of 3H-aspartic acid as the tracer in electron-microscopic autoradiography. The entire process of matrix synthesis and transport takes approximately 2 h. It seems that the protein moiety of the organic matrix is synthesized in the RER prior to 5 min following the initial 10 min incubation in the tracer. At the 5 min chase the label is moving from the RER to the Golgi complexes where the carbohydrate moiety of the matrix is presumed to be synthesized. At the 5 to 15 min chases the label is transported out of the Golgi complexes via Golgi vesicles. This phase continues for 30 min. From 60 to 120 min the 3H-aspartic acid moves to the spicules. After 120 min the majority of the label has moved into the spicules. Silver grain counts over both multivesicular and electron-dense bodies remain at relatively low and constant levels over 4 h indicating that neither organelle is involved in the synthesis and transport of the organic matrix.
Authors:
R J Kingsley; N Watabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Cell and tissue research     Volume:  235     ISSN:  0302-766X     ISO Abbreviation:  Cell Tissue Res.     Publication Date:  1984  
Date Detail:
Created Date:  1984-06-18     Completed Date:  1984-06-18     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0417625     Medline TA:  Cell Tissue Res     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  533-8     Citation Subset:  IM; S    
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MeSH Terms
Descriptor/Qualifier:
Animals
Aspartic Acid / metabolism*
Autoradiography
Biological Transport
Calcification, Physiologic*
Cnidaria / metabolism*,  ultrastructure
Chemical
Reg. No./Substance:
56-84-8/Aspartic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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