Document Detail


Synthesis and study of the fluorescein conjugate of the nucleotide dPTP.
MedLine Citation:
PMID:  11562956     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The synthesis of a fluorescein conjugate on the non-natural base P is described. The ability of the newly synthesised fluorescein--dPTP and of a fluorescein-11-dUTP to compete with the natural nucleotide TTP was also studied. Overall the efficiency of labelling a nucleic acid with a fluorescein moiety was found to be approximately equal.
Authors:
W J Cummins; A L Hamilton; C L Smith; M S Briggs
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Nucleosides, nucleotides & nucleic acids     Volume:  20     ISSN:  1525-7770     ISO Abbreviation:  Nucleosides Nucleotides Nucleic Acids     Publication Date:    2001 Apr-Jul
Date Detail:
Created Date:  2001-09-20     Completed Date:  2002-01-09     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100892832     Medline TA:  Nucleosides Nucleotides Nucleic Acids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1049-51     Citation Subset:  IM    
Affiliation:
Amersham Pharmacia Biotech UK Limited, Amersham Place, Little Chalfont, Buckinghamshire HP7 9NA, England.
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MeSH Terms
Descriptor/Qualifier:
Binding, Competitive
DNA Polymerase I / metabolism
Deoxyuracil Nucleotides / metabolism
Fluoresceins / chemical synthesis,  chemistry*,  metabolism
Nucleotides / chemical synthesis*,  chemistry*,  metabolism
Oxazines / chemistry*,  metabolism
Pyrenes / chemical synthesis*
Pyrimidines / chemistry*,  metabolism
Chemical
Reg. No./Substance:
0/Deoxyuracil Nucleotides; 0/Fluoresceins; 0/Nucleotides; 0/Oxazines; 0/Pyrenes; 0/Pyrimidines; 0/pyrene deoxynucleotide; 126128-35-6/6H,8H-3,4-dihydropyrimido(4,5-c)(1,2)oxazin-7-one; 134344-32-4/fluorescein-11-deoxyuridine triphosphate; EC 2.7.7.-/DNA Polymerase I

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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