| Synthesis of short-/medium-chain-length poly(hydroxyalkanoate) blends by mixed culture fermentation of glycerol. | |
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MedLine Citation:
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PMID: 16004451 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Glycerol was used as a substrate in the bio-production of poly(hydroxyalkanoates) (PHAs) in an effort to establish an alternative outlet for glycerol and produce value-added products. Pseudomonas oleovorans NRRL B-14682 and Pseudomonas corrugata 388 grew and synthesized poly(3-hydroxybutyrate) (P3HB) and medium-chain-length PHA (mcl-PHA) consisting primarily of 3-hydroxydecanoic acid (C(10:0); 44 +/- 2 mol %) and 3-hydroxydodecenoic acid (C(12:1); 31 +/- 2 mol %), respectively, from glycerol at concentrations up to 5% (v/v). Cellular productivity maximized at 40% for P. oleovorans in 5% (v/v) glycerol and 20% for P. corrugata in 2% (v/v) glycerol after 72 h. Increasing the glycerol media concentration from 1% to 5% (v/v) caused a 61% and 72% reduction in the molar mass (M(n)) of the P3HB and mcl-PHA polymers, respectively. Proton-NMR analysis of the glycerol-derived P3HB revealed that the M(n) decrease was the result of esterification of glycerol onto the polymer in a chain terminating position. However, no evidence of glycerol-based chain termination was present in the mcl-PHA. The growth patterns of P. oleovorans and P. corrugata on glycerol permitted their use as mixed cultures to produce natural blends of P3HB and mcl-PHA. By incorporating a staggered inoculation pattern and varying the duration of the fermentations, P3HB/mcl-PHA ratios were achieved that varied from 34:66 to 96:4. |
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Authors:
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Richard D Ashby; Daniel K Y Solaiman; Thomas A Foglia |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biomacromolecules Volume: 6 ISSN: 1525-7797 ISO Abbreviation: Biomacromolecules Publication Date: 2005 Jul-Aug |
Date Detail:
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Created Date: 2005-07-11 Completed Date: 2005-11-14 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100892849 Medline TA: Biomacromolecules Country: United States |
Other Details:
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Languages: eng Pagination: 2106-12 Citation Subset: IM |
Affiliation:
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Eastern Regional Research Center, Agricultural Research Service, U.S. Department of Agriculture, 600 East Mermaid Lane, Wyndmoor, Pennsylvania 19038, USA. rashby@errc.ars.usda.gov |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkanes
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chemical synthesis* Culture Media Fermentation Gas Chromatography-Mass Spectrometry Glycerol / chemistry, metabolism* Magnetic Resonance Spectroscopy Polymers / chemical synthesis* Pseudomonas / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Alkanes; 0/Culture Media; 0/Polymers; 56-81-5/Glycerol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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