| Synthesis of recombinant high density lipoprotein with apolipoprotein A-I and apolipoprotein A-V. | |
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MedLine Citation:
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PMID: 21476871 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Abstract It has been shown that apolipoprotein A-V (apoA-V) over-expression significantly lowers plasma triglyceride levels and decreases atherosclerotic lesion development. To assess the feasibility of recombinant high density lipoprotein (rHDL) reconstituted with apoA-V and apolipoprotein A-I (apoA-I) as a therapeutic agent for hyperlipidemic disorder and atherosclerosis, a series of rHDL were synthesized in vitro with various mass ratios of recombinant apoA-I and apoA-V. It is interesting to find that apoA-V of rHDL had no effect on lipoprotein lipase (LPL) activation in vitro and very low density lipoprotein (VLDL) clearance in HepG2 cells and in vivo. By contrast, LPL activation and VLDL clearance were inhibited by the addition of apoA-V to rHDL. Furthermore, the apoA-V of rHDL could not redistribute from rHDL to VLDL after incubation at 37°C for 30 min. These findings suggest that an increase of apoA-V in rHDL could not play a role in VLDL clearance in vitro and in vivo, which could, at least in part, attribute to the lost redistribution of apoA-V from rHDL to VLDL and LPL binding ability of apoA-V in rHDL. The therapeutic application of rHDL reconstituted with apoA-V and apoA-I might need the construction of rHDL from which apoA-V could freely redistribute to VLDL. |
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Authors:
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Xinbo Zhang; Baosheng Chen |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Biological chemistry Volume: 392 ISSN: 1437-4315 ISO Abbreviation: Biol. Chem. Publication Date: 2011 May |
Date Detail:
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Created Date: 2011-04-11 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9700112 Medline TA: Biol Chem Country: Germany |
Other Details:
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Languages: eng Pagination: 423-9 Citation Subset: IM |
Affiliation:
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National Laboratory of Medical Molecular Biology, Department of Biochemistry and Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100005, China. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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