Document Detail


Synthesis of pyridine derivatives of L-phenylalanine as antisickling reagents.
MedLine Citation:
PMID:  6716398     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Several bicyclic agents composed of L-phenylalanine coupled to various pyridines were synthesized: 2-, 3-, and 4-(L- phenylalanylamino )pyridine. All three compounds at 3 mM gave positive morphological antisickling effects on homozygous SS cells under reduced O2 tension. Studies on two of these compounds, 2- and 3-(L- phenylalanylamino )pyridine, showed that these agents increase the deoxy-HbS solubility ratio, Cs/ Cs0 , by 14% at 20 mM. Observed changes in the mean corpuscular hemoglobin concentration (MCHC) values of treated cells ranged from 4% at 1.3 mM to 15% at 5.6 mM in compound concentration. Very minor lytic activity was found for treated cells, indicating water uptake is responsible for changes in the MCHC. Further, exposure of sickle cells to a 3 mM concentration of these agents also increased by 6- to 7-fold cellular deformability of a treated erythrocyte population as compared to an untreated one at the same total O2 saturation of 47%. These agents demonstrate the potential of bicyclic compounds composed of a common constituent, L-Phe, in the development toward a viable therapeutic agent.
Authors:
J Altman; M Gorecki; M Wilchek; J R Votano; A Rich
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of medicinal chemistry     Volume:  27     ISSN:  0022-2623     ISO Abbreviation:  J. Med. Chem.     Publication Date:  1984 May 
Date Detail:
Created Date:  1984-06-18     Completed Date:  1984-06-18     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9716531     Medline TA:  J Med Chem     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  596-600     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Anemia, Sickle Cell / blood
Antisickling Agents / chemical synthesis*
Erythrocytes / drug effects
Hemoglobin, Sickle / metabolism
Humans
Indicators and Reagents
Solubility
Structure-Activity Relationship
Grant Support
ID/Acronym/Agency:
N01-BH-1-3000/BH/FDA HHS
Chemical
Reg. No./Substance:
0/Antisickling Agents; 0/Hemoglobin, Sickle; 0/Indicators and Reagents; 0/deoxyhemoglobin, sickle

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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