Document Detail


Synthesis of platelet activating factor by ocular tissue from inflamed eyes.
MedLine Citation:
PMID:  2003804     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Platelet activating factors (PAFs) are a family of ether lipids with properties that suggest a major role in inflammation. We have previously implicated PAFs in ocular inflammation based on the inhibition of several rabbit models of iritis with a specific PAF receptor antagonist. We have tested ocular tissues for the ability to synthesize PAF. Iris, ciliary body, cornea, and/or retina were carefully dissected from New Zealand white rabbits, and tissue from four eyes was pooled. Tissues were stimulated with calcium ionophore (10 mumol/L), and supernatants were extracted with chloroform-methanol. Platelet-aggregating activity was found in the chloroform phase in 2 of 9, 1 of 8, 0 of 9, and 3 of 9 studies involving iris, retina, ciliary body, or cornea, respectively. Twenty-four hours after the intravitreal injection of 125 ng of endotoxin, aggregating activity was consistently detectable from supernatants of stimulated iris and ciliary body, occasionally present from stimulated retina but not detectable from cornea. The shape of the aggregation curve resembled that produced by 0.5 to 2.0 ng of authentic PAF. Moreover, the aggregation could be completely inhibited by a PAF receptor antagonist and the aggregating activity chromatographed identically on high-performance liquid chromatography to a PAF standard. These studies indicate that PAF-like activity could be detected from several ocular tissues subsequent to inflammation. Iris, ciliary body, retina, vascular endothelium, and/or leukocytes could each contribute to the presence of this inflammatory mediator.
Authors:
J T Rosenbaum; R S Boney; J R Samples; F H Valone
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Archives of ophthalmology     Volume:  109     ISSN:  0003-9950     ISO Abbreviation:  Arch. Ophthalmol.     Publication Date:  1991 Mar 
Date Detail:
Created Date:  1991-04-15     Completed Date:  1991-04-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  7706534     Medline TA:  Arch Ophthalmol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  410-3     Citation Subset:  AIM; IM    
Affiliation:
Department of Medicine, Oregon Health Sciences University, Portland 97201.
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MeSH Terms
Descriptor/Qualifier:
Animals
Calcimycin
Chromatography, High Pressure Liquid
Ciliary Body / metabolism
Cornea / metabolism
Endotoxins
Eye / metabolism*,  pathology
Female
Inflammation / chemically induced,  metabolism*
Iris / metabolism
Male
Platelet Activating Factor / biosynthesis*
Platelet Aggregation
Rabbits
Retina / metabolism
Grant Support
ID/Acronym/Agency:
AI22141/AI/NIAID NIH HHS; EY 06484/EY/NEI NIH HHS; EY 07784/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
0/Endotoxins; 0/Platelet Activating Factor; 52665-69-7/Calcimycin

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