| Synthesis and oligonucleotide incorporation of fluorescent cytosine analogue tC: a promising nucleic acid probe. | |
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MedLine Citation:
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PMID: 17406622 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The tricyclic cytosine, tC, is a fluorescent base analogue with excellent properties for investigating intrinsic characteristics of nucleic acid as well as interactions between nucleic acids and other molecules. Its unique fluorescence properties and insignificant influence on overall structure and dynamics of nucleic acid after incorporation makes tC particularly interesting in fluorescence resonance energy transfer and anisotropy measurements. We here describe a straightforward synthesis of the standard monomer form of tC for DNA solid-phase synthesis, the tC phosphoramidite, and its subsequent incorporation into oligonucleotides. The total synthesis of the tC phosphoramidite takes approximately 8 days and its incorporation and the subsequent oligonucleotide purification an additional day. |
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Authors:
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Peter Sandin; Per Lincoln; Tom Brown; L Marcus Wilhelmsson |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Nature protocols Volume: 2 ISSN: 1750-2799 ISO Abbreviation: Nat Protoc Publication Date: 2007 |
Date Detail:
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Created Date: 2007-04-04 Completed Date: 2007-06-28 Revised Date: 2008-03-24 |
Medline Journal Info:
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Nlm Unique ID: 101284307 Medline TA: Nat Protoc Country: England |
Other Details:
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Languages: eng Pagination: 615-23 Citation Subset: IM |
Affiliation:
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Department of Chemical and Biological Engineering/Physical Chemistry, Chalmers University of Technology, SE-41296 Gothenburg, Sweden. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cytosine
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chemistry Fluorescent Dyes / chemistry Molecular Structure Nucleic Acid Probes / chemical synthesis*, chemistry* Oligonucleotides / chemistry Organophosphorus Compounds / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Fluorescent Dyes; 0/Nucleic Acid Probes; 0/Oligonucleotides; 0/Organophosphorus Compounds; 0/phosphoramidite; 71-30-7/Cytosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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