| Synthesis of multiplication-stimulating activity (rat insulin-like growth factor II) by rat embryo fibroblasts. | |
| | |
MedLine Citation:
|
PMID: 6681602 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Multiplication-stimulating activity, a family of insulin-like growth factors previously identified in medium conditioned by the BRL-3A rat liver cell line, is also synthesized by third passage cultures of rat embryo fibroblasts (REFs) maintained in serum-free medium. Conditioned serum-free medium from REFs was chromatographed on Sephadex G-75 in 1 m acetic acid, and fractions in the size range of BRL-MSA contained MSA immunoreactivity. A dose-response curve of pooled G-75 fractions was parallel to BRL-MSA standard, and levels in the REF-conditioned medium were 0.5-1 microgram/ml. REF-MSA from the Sephadex G-75 pool was equipotent to BRL-MSA in stimulating [3H]thymidine incorporation into DNA in chick embryo fibroblasts and in stimulating DNA synthesis in REFs, as measured by autoradiography. In receptor binding assays using REFs, chick embryo fibroblasts, Swarm rat chondrosarcoma cells, and rat liver membranes, REF MSA was equal to BRL MSA in competition for binding of [125I]iodo-MSA. REF MSA also behaved identically to BRL MSA in a competitive binding protein assay using binding proteins in rat serum. Further characterization of REF MSA using Bio-Gel P-10 chromatography, followed by high pressure liquid chromatography or polyacrylamide gel electrophoresis, indicated that immunoreactive polypeptides in the fibroblast medium corresponded to BRL MSA II (mol wt, 8700) and BRL MSA III (mol wt, 7100). The amount of REF MSA released into the medium increased linearly over time. Cycloheximide decreased the amount of MSA in the medium, and during a recovery period, the amount of MSA returned nearly to control levels. In summary, rat embryo fibroblasts synthesize MSA which is biologically, immunologically, and chemically identical to MSA produced by the BRL-3A rat liver cell line. |
| | |
Authors:
|
S O Adams; S P Nissley; L A Greenstein; Y W Yang; M M Rechler |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: Endocrinology Volume: 112 ISSN: 0013-7227 ISO Abbreviation: Endocrinology Publication Date: 1983 Mar |
Date Detail:
|
Created Date: 1983-03-24 Completed Date: 1983-03-24 Revised Date: 2007-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0375040 Medline TA: Endocrinology Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 979-87 Citation Subset: AIM; IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Assay Cell Line Chick Embryo Cycloheximide / pharmacology DNA Replication / drug effects Embryo, Mammalian Female Fibroblasts / drug effects, metabolism* Insulin-Like Growth Factor II Kinetics Liver Peptide Biosynthesis* Peptides / pharmacology Pregnancy Rats Rats, Inbred Strains |
| Chemical | |
Reg. No./Substance:
|
0/Peptides; 66-81-9/Cycloheximide; 67763-97-7/Insulin-Like Growth Factor II |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Plasminogen activators in tissues of the immature and estrogen-stimulated rat uterus and in uterine ...
Next Document: Endoscopic removal of choriocarcinoma metastasis in a patient with chronic intestinal hemorrhage.