| Synthesis of leukotriene B and other conjugated triene lipoxygenase products by blood cells of the rainbow trout, Salmo gairdneri. | |
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MedLine Citation:
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PMID: 2540829 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Stimulation of whole blood from rainbow trout with the calcium ionophore, A23187 (20 microM), produced leukotrienes B4 and B5 at concentrations in the range 22-30 ng.ml-1 and 8-24 ng.ml-1, respectively. Their identification and quantification was achieved using reverse-phase high-performance liquid chromatography, combined capillary column gas chromatography-electron capture chemical ionization mass spectrometry and ultraviolet spectroscopy. A number of other lipoxygenase products were also detected, but only partially analysed. The fatty acid composition of the leucocytes, which are presumed to be the site of leukotriene synthesis, was determined by thin-layer and gas-liquid chromatography to enable a comparison of the relative levels of the polyunsaturated fatty acids, which act as substrates for the synthesis of these lipoxygenase products. Arachidonic (20:4(n - 6)), eicosapentaenoic (20:5(n - 3)) and docosahexaenoic (22:6(n - 3)) acids represented approx. 6, 5 and 40%, respectively, of the total fatty acid content. |
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Authors:
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T R Pettitt; A F Rowley; S E Barrow |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1003 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1989 May |
Date Detail:
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Created Date: 1989-06-22 Completed Date: 1989-06-22 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 1-8 Citation Subset: IM |
Affiliation:
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Biomedical and Physiological Research Group, School of Biological Sciences, University College of Swansea, U.K. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Calcimycin / diagnostic use Chromatography, High Pressure Liquid Eicosapentaenoic Acid / analogs & derivatives*, biosynthesis Fatty Acids / blood Gas Chromatography-Mass Spectrometry Leukocytes / metabolism* Leukotriene B4 / biosynthesis* Salmonidae / blood* Trout / blood* |
| Chemical | |
Reg. No./Substance:
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0/Fatty Acids; 1553-41-9/Eicosapentaenoic Acid; 52665-69-7/Calcimycin; 71160-24-2/Leukotriene B4; 80445-66-5/leukotriene B5 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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