Document Detail


Synthesis, isolation, and GC analysis of all the 6,8- to 13,15-cis/trans conjugated linoleic acid isomers.
MedLine Citation:
PMID:  15134147     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Octadecadienoic acids with conjugated double bonds are often referred to as conjugated linoleic acid, or CLA. CLA is of considerable interest because of potentially beneficial effects reported from animal studies. Analysis of CLA is usually carried out by GC elution of FAME. If the presence of low-level isomers is of interest, a complementary technique such as silverion HPLC is also used. These analyses have been hindered by a lack of well-characterized commercially available reference materials. Described here are the synthesis and isolation of selected 6,8- through 13,15-positional CLA isomers, followed by isomerization of these CLA isomers with iodine to produce all the possible cis,cis, cis,trans, trans,cis, and trans,trans combinations. Also present are the GC retention times of the CLA FAME relative to gamma-linolenic acid (6c,9c,12c-octadecatrienoic acid) FAME using a 100-m CP Sil-88 capillary column (Varian Inc., Lake Forest, CA). These data include all the CLA isomers that have been identified thus far in foods and dietary supplements and should greatly aid in the future analysis of CLA in these products.
Authors:
P Delmonte; J A G Roach; M M Mossoba; G Losi; M P Yurawecz
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Lipids     Volume:  39     ISSN:  0024-4201     ISO Abbreviation:  Lipids     Publication Date:  2004 Feb 
Date Detail:
Created Date:  2004-05-11     Completed Date:  2005-02-02     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0060450     Medline TA:  Lipids     Country:  United States    
Other Details:
Languages:  eng     Pagination:  185-91     Citation Subset:  IM    
Affiliation:
US Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, Maryland 20740, USA.
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MeSH Terms
Descriptor/Qualifier:
Chromatography, High Pressure Liquid
Gas Chromatography-Mass Spectrometry*
Hydrogenation
Linoleic Acids, Conjugated / chemistry*,  isolation & purification
Stereoisomerism
alpha-Linolenic Acid / chemistry,  isolation & purification
Chemical
Reg. No./Substance:
0/Linoleic Acids, Conjugated; 463-40-1/alpha-Linolenic Acid

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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