| Synthesis of hydroxytyrosol, 2-hydroxyphenylacetic acid, and 3-hydroxyphenylacetic acid by differential conversion of tyrosol isomers using Serratia marcescens strain. | |
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MedLine Citation:
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PMID: 16076144 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We investigated to develop an effective procedure to produce the potentially high-added-value phenolic compounds through bioconversion of tyrosol isomers. A soil bacterium, designated Serratia marcescens strain, was isolated on the basis of its ability to grow on p-tyrosol (4-hydroxyphenylethanol) as a sole source of carbon and energy. During growth on p-tyrosol, Ser. marcescens strain was capable of promoting the formation of hydroxytyrosol. To achieve maximal hydroxytyrosol yield, the growth state of the culture utilized for p-tyrosol conversion as well as the amount of p-tyrosol that was treated were optimized. The optimal yield of hydroxytyrosol (80%) was obtained by Ser. marcescens growing cells after a 7-h incubation using 2 g/L of p-tyrosol added at the end of the exponential phase to a culture pregrown on 1 g/L of p-tyrosol. Furthermore, the substrate specificity of the developed biosynthesis was investigated using m-tyrosol (3-hydroxyphenylethanol) and o-tyrosol (2-hydroxyphenylethanol) as substrates. Ser. marcescens strain transformed completely m-tyrosol and o-tyrosol into 3-hydroxyphenylacetic acid and 2-hydroxyphenylacetic acid, respectively, via the oxidation of the side chain carbon of the treated substrates. This proposed procedure is an alternative approach to obtain hydroxytyrosol, 2-hydroxyphenylacetic acid, and 3-hydroxyphenylacetic acid in an environmentally friendly way which could encourage their use as alternatives in the search for replacement of synthetic food additives. |
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Authors:
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Noureddine Allouche; Sami Sayadi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of agricultural and food chemistry Volume: 53 ISSN: 0021-8561 ISO Abbreviation: J. Agric. Food Chem. Publication Date: 2005 Aug |
Date Detail:
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Created Date: 2005-08-03 Completed Date: 2005-09-19 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0374755 Medline TA: J Agric Food Chem Country: United States |
Other Details:
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Languages: eng Pagination: 6525-30 Citation Subset: IM |
Affiliation:
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Laboratoire des Bio-Procédés, Centre de Biotechnologie de Sfax B.P K. 3038, Sfax, Tunisia. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Kinetics Phenylacetates / metabolism* Phenylethyl Alcohol / analogs & derivatives*, metabolism Serratia marcescens / metabolism* Soil Microbiology |
| Chemical | |
Reg. No./Substance:
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0/Phenylacetates; 10597-60-1/3,4-dihydroxyphenylethanol; 501-94-0/4-hydroxyphenylethanol; 60-12-8/Phenylethyl Alcohol; 614-75-5/2-hydroxyphenylacetic acid; 621-37-4/3-hydroxyphenylacetic acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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