Document Detail

Synthesis of hapten and production of a monoclonal antibody against a derivative of L-hydroxyproline, a special amino acid in hydrolyzed animal protein.
MedLine Citation:
PMID:  23030435     Owner:  NLM     Status:  In-Data-Review    
China's government has prohibited the addition of simply hydrolyzed animal protein from solid leather waste into milk. The objective of this study was to produce a monoclonal antibody against L-hydroxyproline, a special amino acid in hydrolyzed animal protein. L-hydroxyproline was derivatized with N-acetylsulfanilyl chloride and 5-chlorovaleric acid to synthesize the haptens HP1 and HP2. Then, two immunogens from the two haptens were prepared to produce the antibodies. Results showed that only HP1 was able to stimulate the animal immune system and generate the specific antibody to L-hydroxyproline (as the formation of HP1). The obtained monoclonal antibody from HP1 and the heterologous coating hapten HP2 were incorporated into a competitive indirect enzyme linked immunosorbent assay (ELISA) to determine the antibody's specificity and sensitivity. The IC(50) and the limit of detection for HP1 were 0.16 μg/mL and 0.05 μg/mL respectively. The antibody showed low crossreactivity to parental L-hydroxyproline and showed negligible crossreactivity to D-hydroxyproline and other amino acids. The monoclonal antibody was therefore suitable for the development of an immunoassay to monitor the simply hydrolyzed animal protein from solid leather waste in foodstuffs with L-hydroxyproline as the target analyte.
Li L Wang; Ju X Liu; Jing Liu; Jian P Wang
Related Documents :
24424865 - Diagnosis of melioidosis by means of an elisa detecting antibodies topseudomonas pseudo...
3519015 - A radioimmunoassay for the detection of adrenal autoantibodies.
24751715 - Development of vhh antibodies against dengue virus type 2 ns1 and comparison with monoc...
4347595 - Radioimmunoassay of mammalian type c viral proteins. 3. detection of viral antigen in n...
23680795 - Structure-based non-canonical amino acid design to covalently crosslink an antibody-ant...
1474985 - The effect of sulconazole on the antigenic composition of two dermatophyte species.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of environmental science and health. Part. B, Pesticides, food contaminants, and agricultural wastes     Volume:  48     ISSN:  1532-4109     ISO Abbreviation:  J Environ Sci Health B     Publication Date:  2013  
Date Detail:
Created Date:  2012-10-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7607167     Medline TA:  J Environ Sci Health B     Country:  England    
Other Details:
Languages:  eng     Pagination:  9-15     Citation Subset:  IM    
a College of Veterinary Medicine, Agricultural University of Hebei , Baoding Hebei , China.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  A direct competitive enzyme-linked immunosorbent assay for rapid detection of anilofos residues in a...
Next Document:  Nematicidal activity of terpenoids.