Document Detail

Synthesis of guanosine tetra- and pentaphosphates by the obligately anaerobic bacterium Bacteroides thetaiotaomicron in response to molecular oxygen.
MedLine Citation:
PMID:  422517     Owner:  NLM     Status:  MEDLINE    
Guanosine 5'-diphosphate 3'-diphosphate (ppGpp) and guanosine 5'-triphosphate 3'-diphosphate (pppGpp) were detected in formic acid extracts of air-exposed culutres of Bacteroides thetaiotaomicron. The identification of ppGpp and pppGpp in B. thetaiotaomicron was based on the following results: (i) cochromatography of 32P-labeled hyperphosphorylated nucleotides in two different two-dimensional solvent systems with authentic ppGpp and pppGpp; (ii) incorporation of [3H]guanosine into the putative ppGpp and pppGpp; (iii) alkaline lability; and (iv) resistance, to periodate oxidation. There was a marked increase in the concentration of ppGpp and pppGpp after shift from anaerobic to aerobic conditions, and accumulation of both ppGpp and pppGpp was blocked under these conditions by pretreatment of the culture with rifampin or tetracycline. Growth and incorporation of [3H]guanosine, [3H]tymidine, [14C]succinate, and L-[35S]methionine into macromolecules were inhibited immediately upon exposure to air. The accumulation of ppGpp and pppGpp in B. thetaiotaomicron upon exposure to air may represent a novel signal for synthesis of these compounds.
T L Glass; W M Holmes; P B Hylemon; E J Stellwag
Related Documents :
12731827 - Fuels for urban transit buses: a cost-effectiveness analysis.
8016027 - Asbestos in the air of public buildings: a public health risk?
6823577 - Aplastic anemia and red cell aplasia due to pentachlorophenol.
15727297 - Prediction of extreme ozone levels in barcelona, spain.
25493617 - Time to do something about reproducibility.
22679847 - Partnerships in public health: lessons from knowledge translation and program planning.
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of bacteriology     Volume:  137     ISSN:  0021-9193     ISO Abbreviation:  J. Bacteriol.     Publication Date:  1979 Feb 
Date Detail:
Created Date:  1979-05-23     Completed Date:  1979-05-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  2985120R     Medline TA:  J Bacteriol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  956-62     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Bacterial Proteins / biosynthesis
Bacteroides / drug effects*,  metabolism
Guanine Nucleotides / biosynthesis*
Guanosine Pentaphosphate / biosynthesis*
Guanosine Tetraphosphate / biosynthesis*
Nucleic Acids / biosynthesis
Oxygen / pharmacology*
Rifampin / pharmacology
Tetracycline / pharmacology
Reg. No./Substance:
0/Bacterial Proteins; 0/Guanine Nucleotides; 0/Nucleic Acids; 13292-46-1/Rifampin; 33503-72-9/Guanosine Tetraphosphate; 38918-96-6/Guanosine Pentaphosphate; 60-54-8/Tetracycline; 7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Physiological responses of bacteria to cytochalasin A: effects on growth, transport, and enzyme indu...
Next Document:  650 private psychiatric patients.