| Synthesis and gene transfection efficacies of PEI-cholesterol-based lipopolymers. | |
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MedLine Citation:
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PMID: 18616313 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Nine lipopolymers based on low molecular weight polyethyleneimines (PEI) and cholesterol via an ether linkage between the polymer amine and the cholesterol backbone have been synthesized. Different percentage of cholesterol moieties have been grafted on three types of PEI of molecular weights 800, 1200, and 2000. These lipopolymers were studied for gene transfection activities in HeLa cells. All the lipopolymers were first optimized for enhanced transfection efficacies as coliposomes with DOPE. All lipopolymers are better transfecting agents and highly serum compatible than commercially available PEI-25KDa. Transfection efficacies and serum compatibility of lipopolymers were found to be dependent upon the MW of PEI used for lipopolymer synthesis and percentage of cholesterol grafting on lipopolymers. Cell viability assay showed that PEI-25KDa is highly toxic as compared to all the lipopolymers. Lipopolyplexes were characterized by transmission electron microscopy, which showed the presence of spherical aggregates. |
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Authors:
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Avinash Bajaj; Paturu Kondaiah; Santanu Bhattacharya |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2008-07-11 |
Journal Detail:
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Title: Bioconjugate chemistry Volume: 19 ISSN: 1520-4812 ISO Abbreviation: Bioconjug. Chem. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-20 Completed Date: 2008-10-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9010319 Medline TA: Bioconjug Chem Country: United States |
Other Details:
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Languages: eng Pagination: 1640-51 Citation Subset: IM |
Affiliation:
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Department of Organic Chemistry, Indian Institute of Science, Bangalore 560 012, India. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Cell Survival
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drug effects Cholesterol / chemistry* DNA / metabolism Ethers / chemistry Hela Cells Humans Magnetic Resonance Spectroscopy Molecular Weight Phosphatidylethanolamines / metabolism Polyethyleneimine / chemistry* Polymers / chemical synthesis*, chemistry, metabolism*, toxicity Transfection / methods* |
| Chemical | |
Reg. No./Substance:
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0/1,2-dioleoyl-glycero-3-phosphatidyl ethanolamine; 0/Ethers; 0/Phosphatidylethanolamines; 0/Polymers; 57-88-5/Cholesterol; 9002-98-6/Polyethyleneimine; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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